2000 Fiscal Year Final Research Report Summary
Analysis of regulatory functions of immune system in breast-fed infants by TGF-beta in human milk-Basic study in various tissues of mice.
| Project/Area Number |
10470180
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nara Medical University |
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Principal Investigator |
ISHIZAKA Shigeaki Nara Medical University Medical School, Professor, 医学部, 教授 (90159715)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Masahide Nara Medical University, Medical School Associate Professor, 医学部, 助教授 (50230701)
SAITO Shigeru Toyama Medical and Pharmaceutical University, Medical School, Professor, 医学部, 教授 (30175351)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Transforming growth factor-beta / Cytokine / Intestinal lymphocyte / Natural killer cell / Oral administration / mouse / ELISPOT |
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| Research Abstract |
To investigate the effects of a large amount of transforming growth factor (TGF)-beta in human milk on immune system of breast-fed infants, mice received TGF-beta orally were examined. Interleukin(IL)-15 secretion in mouse intestinal epithelium was increased a short time after oral administration of TGF-beta. The increased IL-15 augmented natural killer (NK) cell activities in intraepithelial lymphocytes of intestine. In addition, the proliferation and activities of dendritic cell(DC) subtype 1 in intestinal intraepithelial lymphocytes were stimulated by IL-15 and subsequently IL-12 secretion from DC1 augmented. TH1 cells stimulated by IL-12 secreted remarkedly interferon(IFN)-gamma, suggesting that the increased IFN-gamma results in the augmentation of NK cell activities. NK cell activities in spleen enhanced several days after the augmentation in intestine.
TGF-beta, which contains in human milk, stimulated IL-15 secretion from intestinal epithelial cells. The increased IL-15 led to NK cell activities and IgA production not only in intestine but in various organs. In conclusion, oral administration of TGF-beta seems to be involved in early protection against infection with enteric or respiratory pathogens.
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[Publications] Ishizaka, S., Takeuchi, H., Kimoto, M., Kanda, S.and Saito, S.: "Fosfomycin, an antibiotic, possessed TGF-beta-like immunoregulatory activities."Int.J.Immunopharmacol.. 20. 765-779 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Toychara, M., Yoshikawa, M., Kawamoto, H., Ueda, S., Kimoto, M., Yamao, J., Fukui, H.and Ishizaka, S.: "In vivo regulation of antibody production system by oral administration of transforming growth factor (TGF)-beta."Digest.Org.Mucos.Immunol.. 35. 57-60 (1998)
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[Publications] Yoshikawa, M., Kanda, S., Ueda, S., Toyohara, M., Takegami, T., Shiroi, K., Ishizaka, S.and Saito, S.: "In vivo immunoregulatory effects of transforming growth factor-beta(TGF-beta)-Effects of oral administration of TGF-beta on cytokine production in intestinal lymphocytes."Digest.org.Mucos.Immunol.. 36. 51-53 (1999)
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「研究成果報告書概要(欧文)」より
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