2001 Fiscal Year Final Research Report Summary
Tumor oxygenation to in crease sensitivity to anti-cancerous drug by using artificial oxygen carrier
| Project/Area Number |
10470247
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Keio University |
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Principal Investigator |
KOBAYASHI Koichi School of Medicine, Keio University Professor, 医学部, 教授 (80051704)
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| Co-Investigator(Kenkyū-buntansha) |
TAJIMA Atsushi School of Medicine, Keio University Assistant, 医学部, 助手 (50276276)
WATANABE Masazumi School of Medicine, Keio University Assistant, 医学部, 助手 (90201227)
HORINOUCHI Hirohisa School of Medicine, Keio University Lecture, 医学部, 講師 (60173647)
SHIGEMATSU Naoyuki Radiology, School of Medicine, Keio University Lecture, 医学部, 助手 (30178868)
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| Project Period (FY) |
1998 – 1999
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| Keywords | artificial oxygen carrier / oxygenations of tumors / drug sensitivity test / increment of sensitivity / chemotherapy |
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| Research Abstract |
Cancer cells are often resistant to anti-cancerous agents. One of the reasons of this phenomenon is cancer cells are hypoxic.
To study if artificial oxygen carriers (AOCs) increases sensitivity of tumor cells to anti-cancerous agents, following experiments were designed.
Yoshida sarcoma cells (LY-80) were inplanted to the back muscle of rats.
Quantative analysis of blood flow in the tumors was performed by microsphere method.
Using Oxyspot method, tissue oxygen pressure of the tumors (PtO_2) was evaluated. The AOCs used were hemoglobin vesicles (HbV), albumin-heme (AH) and perfluorocarbons (PFCs). They were intravenously injected. As control, Ractate Ringer solution and 5% albumin solution were injected. Among AOCs, only AH increased PtO_2 upto 130% of the basal value. In another groups PtO_2 was not increased, probably become they scavenged nitric oxide to cause microvascular contraction to decrease blood flow.
To resolve this mechanism further experiments are needed to study microvascular circulation in the tumors. Also arterial infusion of the AOCs and anti-cancerous drugs must be evaluated.
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