1999 Fiscal Year Final Research Report Summary
Development of gene therapy to myocardium using transvenous gene transfection method.
| Project/Area Number |
10470273
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
SAWA Yoshiki Graduate school of medicine, Osaka university, Lecturer, 医学系研究科, 講師 (00243220)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Motonobu Graduate school of medicine, Osaka university, Assistant professor, 医学系研究科, 助手 (90291442)
FUKUSHIMA Norihide Graduate school of medicine, Osaka university, Assistant professor, 医学系研究科, 助手 (30263247)
OHTAKE Shigeaki Graduate school of medicine, Osaka university, Lecturer, 医学系研究科, 講師 (50243209)
KANEDA Yasufumi Graduate school of medicine, Osaka university, Professor, 医学系研究科, 教授 (10177537)
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| Project Period (FY) |
1998 – 1999
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| Keywords | GENE TRANSFECTION / MYOCARDIAL PROTECTION / HVJ |
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| Research Abstract |
The purposes of this research are estabishment of gene transfer method using hemagglutinating virus of Japan(HVJ)-liposome technique and its application to cardioprotection. In 1998 we successfully transfected the β 2-adrenergic receptor(β2AR) gene and the hepatocyte growth factor(HGF)gene in vivo to the rat heart by intracoronary infusion and obtained the following evidence. 1) β2AR gene transfecton to the hypertrophied rat heart enhances the cardiac response to isoproterenol. 2) HGF gene tranfection to the ischemia-reperfusion heart showed more recovery of cardiac function(LVDP, max. dp/dt, coronary flow) than control. In 1999, to examine the possibility of gene for human, we continued experimentation using canine model. We injected HGF gene complexed with HVJ-liposome directly into the canine ishemic myocardium, and showed significant recovery of regional wall function and blood flow four weeks after administration. The number of the capillary vessels was also increased at HGF injected area. These results indicates the possibility of gene therapy to the patients of ischemic heart disease.
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