1999 Fiscal Year Final Research Report Summary
Differential expression of unknown molecules in neurons after peripheral nerve injury
| Project/Area Number |
10470310
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HIRASAWA Yasusuke Kyoto Prefectural University of Medicine, School of Medicine, Professor, 医学部, 教授 (40079851)
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| Co-Investigator(Kenkyū-buntansha) |
OKAJIMA Seiichiro Kyoto Prefectural University of Medicine, School of Medicine, Instructor, 医学部, 助手 (70305580)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Peripheral Nerve / Nerve Regeneration / Transfection / RLCS / Immunohistochemistry / Immunoelectron Microscopy / PCR / cloning |
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| Research Abstract |
In this study c DNA analysis method, Restriction Landmark c DNA Scanning (RLCS), was applied to elucidate the temporal changes of gene expression in neurons after peripheral nerve injury. The ventral horn (VH) of spinal cord (L3-L5) was dissected at 1, 3, 5, and 7 days after the crush injury of rat sciatic nerves. RCLS profiles were obtained from VH, both from the sciatic nerve crush was performed as well as from the contralateral side where no crush was applied as the control. We compared the profile from the control with five profiles obtained from the VH after injury, and surveyed the temporal change of the expression of these 1991 species of mRNA. 37 of these were shown to change their expression level after injury. 37 genes were classified into 23 patterns. These patterns were classified into three categories ; the continuously up-regulated type (10 species), the transiently up-regulated type (22 species), the down-regulated type (5 species). These complex patterns of gene expression demonstrated after the injury suggest that precious regulation in molecular pathways is required for accomplishing nerve regeneration. Furthermore, the rat homologue of uridine kinase (UK) gene was identified as one of the transiently up-regulated genes. UK mRNA was up-regulated at 7 days after the injury.
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[Publications] Morihara T., Mizoguchi A., Takahashi M., Ozaki S., Tsujihara T., Kawano S., Shirasu M., Ohmukai T., Kitada M., Kimura K., Okajima S., Tamai K., Hirasawa Y., Ide C: "Distribution of synaptosomal associated protein 25 in nerve growth cones and reduction of neurite outgrowth by botulinum neurotoxin a without altering growth cone morphology in dorsal root ganglion neurons and PC-12 cells"Neurosciene. 91(2). 695-706 (1999)
Description
「研究成果報告書概要(欧文)」より
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