1999 Fiscal Year Final Research Report Summary
APPLICATION OF BGL-2 ANTISENSE OLIGO-DNA FOR GENE THERAPY TO UROLOGICAL CANCER.
| Project/Area Number |
10470333
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
TOMITA Yoshihiko Faculty of Medicine, NIIGATA UNIVERSITY associate, 医学部, 助教授 (90237123)
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| Co-Investigator(Kenkyū-buntansha) |
TANIKAWA Toshiki University Hospital, NIIGATA UNIVERSITY Lecturer, 医学部・附属病院, 講師 (70236686)
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| Project Period (FY) |
1998 – 1999
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| Keywords | renal cell cancer / bcl-2 / antisense oligonucleotide / gene therapy |
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| Research Abstract |
We have previously shown that Bcl-2 expression was negative prognostic factor in transitional cell cancer (TCC), and that TCC cell lines expressing high levels of Bcl-2 are resistant to Adriamycin triggered apoptosis. Here we examined antisense oligonucleotide - mediated downregulation of Bcl-2 expression and its effect on sensitivity to ADM treatment in T24 cells. Treatment of T24 cells with 20 μM of bcl-2 antisense PODN reduced the Bcl-2 protein level. Combined administration with Adriamycin resulted in synergistic cytotoxicity, accompanied with a 2.4-fold increase in DEVD-specific caspase activity. The finding provides evidence that Bcl-2 expression may be critical for maintaining the drug resistance of TCC. bcl-2 antisense PODN might be useful means for. overcoming drug resistance in highly malignant TCC,
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Research Products
(14 results)
