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2000 Fiscal Year Final Research Report Summary

The correlation between chromosome aberrations and head morphology or motility of human spermatozoa : Analysis by using intracytoplasmic sperm injection into mouse oocytes

Research Project

Project/Area Number 10470339
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionAsahikawa Medical College

Principal Investigator

KAMIGUCHI Yujiroh  Asahikawa Med.Col., Dept.Biol.Sci., Professor, 医学部, 教授 (60091568)

Co-Investigator(Kenkyū-buntansha) SEIJI Watanabe  Asahikawa Med.Col., Dept.Biol.Sci., Research Associate, 医学部, 教務職員 (10241449)
TATENO Hiroyuki  Asahikawa Med.Col., Dept.Biol.Sci., Associate Professor, 医学部, 助教授 (80163492)
Project Period (FY) 1998 – 2000
KeywordsHuman spermatozoa / Chromosome aberrations / Mouse oocytes / intracytoplasmic sperm injection
Research Abstract

(1) A highly successful method for human sperm chromosome analysis was established by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Namely, 7O.7% of sperm-injected oocytes were able to reach the first cleavage metaphase, and 94.6% of metaphasic ova were successfully karyotyped.
(2) In vitro aging of mouse oocytes and human spermatozoa, which takes place when they are waited for ICSI in the culture medium, may be associated with the incidence of sperm chromosome aberrations. However, no change was found in the aberration frequency even when both gametes were aged in vitro for about 3 hours.
(3) In motile human spermatozoa with normal morphology, the incidences of aneuploidy and structural chromosome aberrations were 1.7% and 8.8% respectively. These incidences were not statistically different between fresh and frozen-thawed spermatozoa, indicating that cryopreservation of spermatozoa in liquid nitrogen has no deleterious effect on sperm chromosomes.
(4) No increase of the chromosome aberrations was found in the spermatozoa with a large head, a small head, and a pointed head, as compared with the aberration frequency in morphologically normal spermatozoa. In contrast, the frequency was significantly higher in the spermatozoa with an elongated head.
(5) The incidence of structural chromosome aberrations in immotile spermatozoa with normal morphology was similar to that in motile spermatozoa when immotile spermatozoa were kept in seminal fluid until ICSI.On the contrary, aberration frequency increased significantly when immotile spermatozoa were stored in the culture media (BWW, HTF) for 2 hours or more. An increasing tendency was found in PBS-stored spermatozoa. Aberration frequency was also heightened by centrifugation of spermatozoa.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Tateno,Hiroyuki: "Dithiothreitol induces sperm nuclear decondensation and protects against chromosome damage during male pronuclear formation in hybrid zygotes between Chinese hamster spermatozoa and Syrian hamster oocytes"Zygote. 7. 321-327 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邉誠二: "マウス卵へのICSI法を用いたヒト精子染色体分析法の確立および卵子の加齢と精子染色体異常の関連性の検討"日本不妊学会雑誌. 44(3). 290 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邉誠二: "ICSI法によりマウス卵に授精させたヒト形態正常精子の染色体異常"日本不妊学会雑誌. 44(4). 418 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateno,Hiroyuki: "Sonication per se is not as deleterious to sperm chromosomes as previously inferred"Biology of Reproduction. 63. 341-346 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邉誠二: "マウス卵への細胞質内注入(ICSI)法を利用した頭部形態異常ヒト精子の染色体分析"Journal of Mammalian Ova Research. 17(2). S28 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邉誠二: "ICSI法によりマウス卵に授精させたヒト形態異常精子および不動精子の染色体異常"日本不妊学会雑誌. 45(4). 546 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邉誠二: "マウス卵への細胞質内精子注入(ICSI)法を用いたヒト精子染色体研究法の確立"旭川医科大学研究フォーラム. 2(1)(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateno, Hiroyuki et al.: "Dithiothreitol induces sperm nuclear decondensation and protects against chromosome damage during male pronuclear formation in hybrid zygotes between Chinese hamster spermatozoa and Syrian hamster oocytes"Zygote. 7. 321-327 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Seiji et al.: "Establishment of a method of human sperm chromosome analysis by using intracytoplasmic sperm injection (ICSI) into mouse oocytes, and examination of a possible correlation between oocyte aging and sperm chromosome aberrations"Japanese Journal of Fertility and Sterility. 44(3). 290 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Seiji et al.: "Chromosome aberrations in morphologically normal human spermatozoa fertilized with mouse oocytes by using ICSI method"Japanese Journal of Fertility and Sterility. 44(4). 418 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateno, Hiroyuki et al.: "Sonication per se is not as deleterious to sperm chromosomes as previously inferred"Biology of Reproduction. 63. 341-346 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Seiji et al.: "Chromosome analysis of human spermatozoa with morphologically abnormal head by using intracytoplasmic sperm injection (ISCI) into mouse oocytes"Journal of Mammalian Ova Research. 17(2). S28 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Seiji et al.: "Chromosome aberrations in morphologically abnormal or immotile human spermatozoa fertilized with mouse oocytes by using ICSI method"Japanese Journal of Fertility and Sterility. 45(4). 546 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Seiji et al.: "Establishment of a method for human sperm chromosome study by using intracytoplasmic sperm injection (ICSI) into mouse oocytes"Asahikawa Medical College Research Bulletin. 2(1) (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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