2001 Fiscal Year Annual Research Report

気道腺組織の分化と増殖についての研究

Research Project

Project/Area Number	10470355
Research Institution	Mie University
Principal Investigator	間島雄一三重大学, 医学部, 教授 (60024791)
Co-Investigator(Kenkyū-buntansha)	清水猛史三重大学, 医学部・附属病院, 助教授 (00206202) 原田輝彦三重大学, 医学部, 助教授 (20183569)
Keywords	腺細胞 / 気道液 / 慢性副鼻腔炎 / 増殖 / 分化 / 過形成 / 増殖因子 / 動物モデル
Research Abstract	本年度と過去4年間の研究成果の概要を示す。腺細胞の3D培養は無血清倍地での培養に成功し、これを用いて増殖因子(EGF,KGF)およびビタミンA誘導体の培養腺細胞の増殖と分化に及ぼす影響を明らかにした。培養腺細胞のムチン産生についても検討し、LPS刺激によりMUC2,MUC5ACの発現は増強されたがMUC5Bに変化をみなかった。ラット気管粘液産生モデルを用いて薬物の粘液産生量に及ぼす影響を影響を定量的測定し、ステロイド,14員環マクロライドの粘液産生抑制作用を明らかにした。また、鼻・副鼻腔患者における粘液産生に及ぼす影響を検討し、粘液産生が、急性,慢性炎症さらにI型アレルギー反応により産生される場合にいかなる相違が存在するかを検討した。また鼻・副鼻腔の病的状態で産生された粘液がどのような物理学的性質を有しており、その性質は何により決定されるかを明らかにした。結論として鼻・副鼻腔の慢性炎症では主として腺細胞からの粘液産生が亢進しており、この原因は増殖因子による腺の増殖と粘液産生を亢進する種々の炎症産物によるものと考えられる。腺の増殖に関与する因子は他にも存在する可能性があり、今後はさらにこれら因子の検討を行い、臨床において腺の過分泌抑制を可能にしたい。

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Majima, Y., et al.: "Effect of biochemical components on rheologic properties of nasal mucus in chronic sinusitis"Am J Respir Crit Care Med. 160. 421-426 (1999)
[Publications] Rhee, CS., et al.: "Effects of mucokinetic drugs on rheological properties of reconstituted human nasal mucus"Arch Otolaryngol Head Neck Surg. 125. 101-105 (1999)
[Publications] Rhee, CS., et al.: "Effects of clarithromycin on rheological properties of nasal mucus in patients with chronic sinusitis"Ann Otol Rhinol Laryngol. 109. 485-487 (2000)
[Publications] Fujita, K., et al.: "Effects of macrolides on interleukin-8 secretion from human nasal epithelial cells"Eur Arch Otorhinolaryngology. 257. 199-204 (2000)
[Publications] Shimizu, T., et al.: "Mechanism of antigen-induced mucus production in nasal epithelium of sentitized rats.A comparison with lipopolysaccharide-induced mucus production"Am J Respir Crit Care Med. 161. 648-1654 (2000)
[Publications] Shimizu, T., et al.: "Role of neutrophil elastase in endotoxin-induced mucus hypersecretion in rat nasal epithelium"Ann Otol Rhinol Laryngol. 109. 1049-1054 (2000)
[Publications] Nonoyama, T., et al.: "Immunohistochemical localization of cytokines and cell adhesion molecules in maxillary sinus mucosa in chronic sinusitis"Auris Nauris Larynx. 27. 51-58 (2000)
[Publications] Takeuchi, K., et al.: "Tumor necrosis factor gene polymorphis in chronic sinusitis"Laryngoscope. 110. 1711-1714 (2000)
[Publications] Takeuchi, K., et al.: "The V δ T cell receptor repertoire in human nasal mucosa"Scand J Immunol. 52. 380-384 (2000)
[Publications] Usui, S., et al.: "Secretory cell differentiation and mucus secretion in cultures of human nasal epithelial cells:Use of a monoclonal antibody to study human nasal mucin"Ann Otol Rhinol Laryngol. 109. 271-277 (2000)
[Publications] Shimizu, T., et al.: "Differential properties of mucous glycoproteins in rat nasal epithelium.A comparison between allergic inflammation and lipopolysaccharide stimulation"Am J Respir Crit Care Med. 164. 1077-1082 (2001)
[Publications] Shinogi, J., et al.: "Quantitative analysis of mucin and lectin in maxillary sinus fluids in patients with acute and chronic sinusitis"Laryngoscope. 111. 240-245 (2001)
[Publications] Kimura, T., et al.: "The effect of growth factors on human nasal glands proliferation and differentiation"Arch Otolaryngology Head Neck Surg.. (掲載予定).

2001 Fiscal Year Annual Research Report

気道腺組織の分化と増殖についての研究

Principal Investigator

間島 雄一 三重大学, 医学部, 教授 (60024791)

Research Products

[Publications] Majima, Y., et al.: "Effect of biochemical components on rheologic properties of nasal mucus in chronic sinusitis"Am J Respir Crit Care Med. 160. 421-426 (1999)

[Publications] Rhee, CS., et al.: "Effects of mucokinetic drugs on rheological properties of reconstituted human nasal mucus"Arch Otolaryngol Head Neck Surg. 125. 101-105 (1999)

[Publications] Rhee, CS., et al.: "Effects of clarithromycin on rheological properties of nasal mucus in patients with chronic sinusitis"Ann Otol Rhinol Laryngol. 109. 485-487 (2000)

[Publications] Fujita, K., et al.: "Effects of macrolides on interleukin-8 secretion from human nasal epithelial cells"Eur Arch Otorhinolaryngology. 257. 199-204 (2000)

[Publications] Shimizu, T., et al.: "Mechanism of antigen-induced mucus production in nasal epithelium of sentitized rats.A comparison with lipopolysaccharide-induced mucus production"Am J Respir Crit Care Med. 161. 648-1654 (2000)

[Publications] Shimizu, T., et al.: "Role of neutrophil elastase in endotoxin-induced mucus hypersecretion in rat nasal epithelium"Ann Otol Rhinol Laryngol. 109. 1049-1054 (2000)

[Publications] Nonoyama, T., et al.: "Immunohistochemical localization of cytokines and cell adhesion molecules in maxillary sinus mucosa in chronic sinusitis"Auris Nauris Larynx. 27. 51-58 (2000)

[Publications] Takeuchi, K., et al.: "Tumor necrosis factor gene polymorphis in chronic sinusitis"Laryngoscope. 110. 1711-1714 (2000)

[Publications] Takeuchi, K., et al.: "The V δ T cell receptor repertoire in human nasal mucosa"Scand J Immunol. 52. 380-384 (2000)

[Publications] Usui, S., et al.: "Secretory cell differentiation and mucus secretion in cultures of human nasal epithelial cells:Use of a monoclonal antibody to study human nasal mucin"Ann Otol Rhinol Laryngol. 109. 271-277 (2000)

[Publications] Shimizu, T., et al.: "Differential properties of mucous glycoproteins in rat nasal epithelium.A comparison between allergic inflammation and lipopolysaccharide stimulation"Am J Respir Crit Care Med. 164. 1077-1082 (2001)

[Publications] Shinogi, J., et al.: "Quantitative analysis of mucin and lectin in maxillary sinus fluids in patients with acute and chronic sinusitis"Laryngoscope. 111. 240-245 (2001)

[Publications] Kimura, T., et al.: "The effect of growth factors on human nasal glands proliferation and differentiation"Arch Otolaryngology Head Neck Surg.. (掲載予定).

間島雄一三重大学, 医学部, 教授 (60024791)