2000 Fiscal Year Annual Research Report

鼻副鼻腔粘膜の免疫アレルギー疾患にかかわるT細胞を標的とした治療の開発-分子生物学的手法を用いたヒト鼻粘膜T細胞の解析-

Research Project

Project/Area Number	10470356
Research Institution	SHIMANE MEDICAL UNIBERSITY
Principal Investigator	川内秀之島根医科大学, 医学部, 教授 (50161279)
Co-Investigator(Kenkyū-buntansha)	佐野啓介島根医科大学, 医学部, 助手 (10263542) 片岡真吾島根医科大学, 医学部, 助手 (60152667) 加藤太二島根医科大学, 医学部, 助教授 (20185846)
Keywords	鼻粘膜リンパ球 / サイトカイン / RT-PCR / アレルギー性鼻炎 / 慢性副鼻腔炎 / フローサイトメトリー / ヘルパーT細胞 / 抑制性T細胞
Research Abstract	1.アレルギー性鼻炎あるいは慢性副鼻腔炎患者鼻腔粘膜より採取した鼻粘膜リンパ球を用いて、フローサイトメトリーを行い、T細胞分画の解析を行った。その結果、種々の割合でCD4+ヘルパーT細胞ならびにCD8+抑制性T細胞が分布していることが示された。 2.PRISM7700sequence detectorを使用して、鼻腔粘膜より得られた少量のリンパ球を用いて、定量的なRT-PCRを行い、Th1タイプ(IFN-gamma,IL-2)およびTh2タイプ(IL-4,IL-5)のサイトカインについてmRNAでのサイトカインプロフィールについて検討した。その結果、アレルギー性鼻炎ならびに慢性副鼻腔炎患者においては、IFN-gammaの発現は見られたものの、Th2タイプのIL-4やIL-5については、さらにin vitroでアレルゲン刺激を行うことにより、その発現が確認できた。 3.アレルギー性鼻炎患者と慢性副鼻腔炎患者の鼻粘膜リンパ球のサイトカインプロフィールの違いについて検討し、アレルギー性鼻炎ではTh2タイイトカインがより強く発現されている傾向が見られた。Th1タイプのサイトカインについては、採取したばかりの鼻粘膜リンパ球では両疾患患者の間に有意な差はなかったが、in virtoでアレルゲン刺激を一定の時間加えたのちに、IFN-gammaの産生がむしろアレルギー性鼻炎患者で増強する傾向にあった。 4.鼻粘膜から得られる少量のリンパ球を用いて、細胞内サイトカインを蛋白レベルで測定するサイトカインフローサイトメトリーに関しては、未だアッセイシステムを確立するに至っていない。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] 柴宏巳: "ヒト鼻咽腔粘膜における抗原提示細胞の分布"耳鼻咽喉科免疫アレルギー. 16・1. 21-25 (1998)
[Publications] Tomoko Nagata: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins."Biochimica et Biophysica Acta. 1401. 319-328 (1998)
[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み:NOD-scidマウスを用いた検討"口咽科. 10・2. 149-160 (1998)
[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み"日本耳鼻咽喉科感染症研究会会誌. 16・1. 60-66 (1998)
[Publications] Hideyuki Kawachi: "The role of tonsils of as an Inductive site of Immune response in nasopharynx,"Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 83-87 (1998)
[Publications] Hideyuki Kawachi: "Mucosal immunity of nasopharynx."Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 89-93 (1998)
[Publications] Chiaki Sano: "Therapeutic Effects of Benzoxazinorifamycin KRM-1648 Secretory Leukocyte Protease Inhibitor or the Nonsteroidal Anti-Inflammatory Drug Diclofenac Sodium against Mycobacterium avium Complex Infection in Mice."Antimicrobial Agents And Chemotherapy. 43・2. 360-346 (1999)
[Publications] Chiaki Sano: "The modulating effects of proinflammatory cytokines, interferon-γ and tumor necrosis factor-α and immunoregulating cytokines, IL-10 and transforming growth factor-β, on antimicrobial activity of murine peritoneal macrphages against Mycobacterium avium-intracellulare"Clinical and Experimental Immunology. 115・3. 435-442 (1999)
[Publications] 川内秀之: "ヘルパーT細胞と調節性(抑制性)T細胞の今昔."耳鼻咽喉科免疫アレルギー. 17・1. 51-52 (1999)
[Publications] Tomoyuki Nishikori: "Apoptosis during inner ear development in human and mouse embryos : an analysis by computer-assisted three-dimensional reconstruction."Anat Embryol. 200. 19-26 (1999)
[Publications] Masashi Suzuki: "Efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis -An experimental study-."Annals of Otology, Rhinology & Laryngology. 108・6. 554-558 (1999)
[Publications] Masashi Suzuki: "Clinical efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis."Auris.Nasus.Larynx.. 26・2. 123-129 (1999)
[Publications] Hideyuki Kawauchi: "Mucosal immunity of nasopharynx -an experimental study in TCR-transgenic (OVA23-3) mice."Rhinology Supplement. 15. 56 (1999)
[Publications] Nobue Uchida: "Squamous cell carcinoma of the external auditory canal -Two cases treated with high dose rate^<192> Ir remote afterloading system (RALS)."Reprinted from Radiation Medicine. 17・6. 443-446 (1999)
[Publications] Hideyuki Kawauchi: "T-cell mucosal imminity in the nasopharynx."New Frontiers in Immunobiology. 35-41 (2000)
[Publications] 川内秀之: "上気道の生体防御機構と炎症性疾患の病態と治療."耳鼻咽喉科展望. 43・1. 10-22 (2000)
[Publications] Hisashi Ohga: "S-carboxymethylCysteine (S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils."Recent Advances in Otitis Media. (in press).
[Publications] Ryoutaro Ishimitsu: "Overexpression of IL-15 In Vivo Enhances Tc1 Response, Which Inhibits Allergic Inflammation in a Murine Model of Asthma"The Journal of Immunology. 166. 1991-2001 (2001)
[Publications] Ryoutaro Ishimitsu: "Dichotomous effect of a tranditional Japanese medicine, Bu-zhong-yi-qi-tang on allergic asthma in mice."Immunopharmacology. (in press).

2000 Fiscal Year Annual Research Report

鼻副鼻腔粘膜の免疫アレルギー疾患にかかわるT細胞を標的とした治療の開発-分子生物学的手法を用いたヒト鼻粘膜T細胞の解析-

Principal Investigator

川内 秀之 島根医科大学, 医学部, 教授 (50161279)

Research Products

[Publications] 柴宏巳: "ヒト鼻咽腔粘膜における抗原提示細胞の分布"耳鼻咽喉科免疫アレルギー. 16・1. 21-25 (1998)

[Publications] Tomoko Nagata: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins."Biochimica et Biophysica Acta. 1401. 319-328 (1998)

[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み:NOD-scidマウスを用いた検討"口咽科. 10・2. 149-160 (1998)

[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み"日本耳鼻咽喉科感染症研究会会誌. 16・1. 60-66 (1998)

[Publications] Hideyuki Kawachi: "The role of tonsils of as an Inductive site of Immune response in nasopharynx,"Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 83-87 (1998)

[Publications] Hideyuki Kawachi: "Mucosal immunity of nasopharynx."Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 89-93 (1998)

[Publications] Chiaki Sano: "Therapeutic Effects of Benzoxazinorifamycin KRM-1648 Secretory Leukocyte Protease Inhibitor or the Nonsteroidal Anti-Inflammatory Drug Diclofenac Sodium against Mycobacterium avium Complex Infection in Mice."Antimicrobial Agents And Chemotherapy. 43・2. 360-346 (1999)

[Publications] 川内秀之: "ヘルパーT細胞と調節性(抑制性)T細胞の今昔."耳鼻咽喉科免疫アレルギー. 17・1. 51-52 (1999)

[Publications] Tomoyuki Nishikori: "Apoptosis during inner ear development in human and mouse embryos : an analysis by computer-assisted three-dimensional reconstruction."Anat Embryol. 200. 19-26 (1999)

[Publications] Masashi Suzuki: "Efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis -An experimental study-."Annals of Otology, Rhinology & Laryngology. 108・6. 554-558 (1999)

[Publications] Masashi Suzuki: "Clinical efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis."Auris.Nasus.Larynx.. 26・2. 123-129 (1999)

[Publications] Hideyuki Kawauchi: "Mucosal immunity of nasopharynx -an experimental study in TCR-transgenic (OVA23-3) mice."Rhinology Supplement. 15. 56 (1999)

[Publications] Nobue Uchida: "Squamous cell carcinoma of the external auditory canal -Two cases treated with high dose rate^<192> Ir remote afterloading system (RALS)."Reprinted from Radiation Medicine. 17・6. 443-446 (1999)

[Publications] Hideyuki Kawauchi: "T-cell mucosal imminity in the nasopharynx."New Frontiers in Immunobiology. 35-41 (2000)

[Publications] 川内秀之: "上気道の生体防御機構と炎症性疾患の病態と治療."耳鼻咽喉科展望. 43・1. 10-22 (2000)

[Publications] Hisashi Ohga: "S-carboxymethylCysteine (S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils."Recent Advances in Otitis Media. (in press).

[Publications] Ryoutaro Ishimitsu: "Overexpression of IL-15 In Vivo Enhances Tc1 Response, Which Inhibits Allergic Inflammation in a Murine Model of Asthma"The Journal of Immunology. 166. 1991-2001 (2001)

[Publications] Ryoutaro Ishimitsu: "Dichotomous effect of a tranditional Japanese medicine, Bu-zhong-yi-qi-tang on allergic asthma in mice."Immunopharmacology. (in press).

川内秀之島根医科大学, 医学部, 教授 (50161279)