2001 Fiscal Year Final Research Report Summary
Synaptic relationship between primary, afferent newe terminals and interneurons in the spinal trigeminal nucleus caudalis
| Project/Area Number |
10470382
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HIURA Akio The University of Tokushima, School of Dentistry, Associate Prof., 歯学部, 助教授 (00106353)
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| Co-Investigator(Kenkyū-buntansha) |
KUWAHARA Michiyo The University of Tokushima, School of Dentistry, Assist., 歯学部, 助教授 (70243706)
NASU Fumio The University of Tokushima, School of Dentistry, Assist., 歯学部, 助手 (10180530)
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| Project Period (FY) |
1998 – 2001
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| Keywords | Synapses / Primary afferent neuron / Substantia gelatinosa / Interneuron / Neurophysiological study |
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| Research Abstract |
The aim of this study is to identify the interneurons responding to the electrical stimuli peculiar to convey nociceptive information in the substantia gelatinosa of the mouse superficial dorsal horn. The second is to, investigate the synaptic relation of nociceptive primary afferent nerve (PAN) terminals to inhibitory interneurons such as GABAergic, thereby prove the flow of impulse from the PAN terminals to GABAergic interneurons. Taking out the spinal cord or medulla oblongata, the specimens were coronally cut at 500 μm thickness with microslicer and put into the chamber continuously circulated with medium (PH7.2 〜 7.4) adjusted the temperature at 37℃, including 95 % O_2 and 5 % CO_2.
Under the above ideal conditions, the glass electrode was inserted into the substantia gelatinosa. But now the resting potentials of the interneurons could not be obtained. Probably, the neurons were dying during the experiment. Thus, we have no data in this study, however, we have at least prepared the circumferential conditions to carry out the study. We should continue this study, overcome the problems and would get some of the interesting results in near future.
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