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1999 Fiscal Year Final Research Report Summary

Effect of mechanical stress on chondrocytes metabolism

Research Project

Project/Area Number 10470415
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KUBOKI Takuo  Okayama University, Dental Hospital, Lecture, 歯学部・附属病院, 講師 (00225195)

Co-Investigator(Kenkyū-buntansha) HATTORI Takako  Okayama University, Dental School, Research Associate, 歯学部, 助手 (00228488)
TAKIGAWA Masaharu  Okayama University, Dental School, Professor, 歯学部, 教授 (20112063)
Project Period (FY) 1998 – 1999
KeywordsChondrocyte / Cartilage Degradation Factors / Cyclic Mechanical Stress / Matrix Metalloproteinases / Interleukin-1 / Proteoglycan
Research Abstract

To clarify the mechanism of cartilage degradation induced by mechanical stress, we investigated the influence of cyclic tension force (CTF) on the metabolism of cultured chondrocytes, The chondrocytes were exposed to CTF by using Flexercell strain unit. Five or 15 kPa of high frequency CTF significantly inhibited the syntheses of DNA, proteoglycan, collagen and protein. Fifteen kPa of high frequency CTF induced the expression of interleukin-1 (IL-1), matrix metalloproteinase (MMP)-2 and -9 mRNA, and increased the production of pro-and active-Mmp-9. the degradation of proteoglycan was inhibited by MMP Inhibitor, indicating that MMPs are involved in the degradation of proteoglycan induced by high frequency CTF.
Moreover reducing the frequency of CTF from high to low decreased the inhibition of proteoglycan synthesis. These findings suggest that the CTF frequency is one of the key determinants of the chondrocyte metabolism. Low magnitude CTF, whether high or low frequency, did not cause the gene expression of cartilage degradation factors, suggesting that this magnitude of CTF causes only minor change of cartilage matrix. High magnitude and frequency CTF caused the gene expression of IL-1 and MMP-9, followed by increases in the production of MMP-2 and -8 protein suggest that excessive and continuous cyclic mechanical stress induces the production of IL-1 and MMP-9, resulting in cartilage degradation.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] M. Kanyama, S. Kojima, T. Fujisawa, T. Kuboki, H. Yatani, and A. Yamashita: "MMPs and TIMPs in synovial fluids of TMJ osteoarthritis patients"J. Dent. Res.. 77. 1004 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Fujisawa, T. Hattori, T. Kuboki, A. Yamashita and M. Takigawa: "Effect of cyclic mechanical stress on chondrocyte metabolism"J. Dent. Res.. 77. 1004 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Fujisawa, T. Hattori, T. Kuboki, A. Yamashita and M. Takigawa: "Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metallproteinases and interleukin-1"J. Biochem.. 125. 966-975 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Kuboki, T. Hattori, T. Mizushima, M. Kanyama, T. Fujisawa, A. Yamashita and M. Takigawa: "Ditection specific antibodies against human cultured chondrosarcoma (HCS-2.8) and osterosarcoma (Saos-2) cells in the serum of patients with osteoarthritis of temporo-mandibular joint"Arch. Oral Biol.. 44. 403-414 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Kuboki, T. Nakanishi, M. Kanyama, W. Sonoyama, T. Fujisawa, K. Kobayashi, T. Ikeda, T, Kubo, A. Yamashita and M. Takigawa: "Direct adenovirus-mediated gene delivery to the temporo-mandibular joint in guinea pigs"Arch. Oral Biol.. 44. 701-709 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Kanyama, T. Kuboki, S. Kojima, T. Fujisawa, T. Hattori, M. Takigawa, and A. Yamashita: "Matrix metalloproteinases and tissue Inhibitors of metalloproteinases in synovial fluids of temporomandibular joint osteoarthritis patients"J Orofac Pain. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Kanyama, S. Kojima, T. Fujisawa, T. Kuboki, H. Yatani and A. Yamashita: "MMPs and TIMPs in synovial fluids of TMJ osteoarthritis patients"J. Dent Res.. 77. 1004 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Fujisawa, T. Hattori, T. Kuboki, A. Yamashita and M. Takigawa: "Effect of cyclic mechanical stress on chondrocyte metabolism"J. Dent. Res,. 77. 1004 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Fujisawa, T. Hattori, K. Takahashi, T. Kuboki and M. Takigawa: "Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interlu\eukin-1"J. Biochem. 125. 966-975 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kuboki, T. Hattori, T. Mizushima, M. Kanyama, T. Fujisawa, A. Yamashita and M. Takigawa: "Ditection specific antibodies against human cultured chondrosarcoma (HCS-2/8) and osteosarcoma (Saos-2) cells in the serum of patients with osteoarthritis of temporo-mandibular joint"Arch. Oral Biol.. 44. 403-414 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kuboki, T. Nakanishi, M. Kanyama, W. Sonoyama, T. Fujisawa, K. Kobayashi, T. Ikeda, T. Kubo, A. Yamashita and M. Takigawa: "Direct adenovirus-mediated gene delivery to the temporo-mandibular joint in guinea pigs"Arch. Oral Biol.. 44. 701-709 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Kanyama, T. Kuboki, S. Kojima, T. Fujisawa, T. Hattori, M. Takigawa, A. Yamashita: "Matrix metalloproteinases and tissue Inhibitors of metalloproteinases in synovial fluids of temporomandibular joint osteoarthritis patients"J Orofac Pain. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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