1999 Fiscal Year Final Research Report Summary
Cloning and Characterization of the Hemifacial Microsomia Related Gene.
| Project/Area Number |
10470456
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Shimane Prefectural Shimane Women's College |
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Principal Investigator |
NAORA Hiroyuki Shimane Women's College, Food Science, Instructor, 家政科, 助手 (70222156)
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| Co-Investigator(Kenkyū-buntansha) |
OTANI Hiroki Shimane Medical University, Medicine, Professor, 医学部, 教授 (20160533)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Hemifacial Microsomia / Craniofacial Anomaly / Insertional Mutation / Chromosome 10 / Animal Model / Transgenic Mouse / 遺伝子導入マウス |
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| Research Abstract |
To get an insight into the genetical predisposition and pathogenesis of the hemifacial microsomia (HFM), we analyzed the mutant locus of the HFM model mouse. By the P1 phage library screening, we isolated the chromosomal DNA from the mutant region. By the DNA sequencing, we screened the coding regions that corresponded to the mutant gene(s). At present, we sequenced about 10 kb, then we identified some regions that had highly homologous sequences with those of some known genes. These genes locate the different loci with HFM locus, then it is suggested that the gene(s) that responds to HFM pathogenesis is a nobel gene, and shares some domain structures to the known genes.
We investigated the phenotype of homo-zygote of HFM mouse. By the natural mating between the hemi zygotes, we obtained 12 embryos at E9.5. Three embryos died as the rosy mass. PCR analysis showed that one of three embryos was a homo-zygote (another two embryos could not determine their genotypes due to DNA degradation). According to the size of the placenta, homo-zygotes embryo developed normally to E7.5. These results showed that the gene that disrupted in HFM mouse had an essential role for the normal development.
The pathological screening was carried out at the late prenatal days (E13.5-17.5). In addition to the branchial arch anomalies that described previously, other anomalies and asymmetries at the maxilla, mandible, zygoma and facial soft tissue ware identified. These observation further supported that HFM mouse is a suitable animal model for the human HFM at the point of the pathological characteristics.
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Research Products
(15 results)
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[Publications] Isao Oishi, Shigeto Takeuchi, Ryuju Hashimoto, Akira Nagabukuro, Takahiro Ueda, Zhao-Jun Liu, Toshihisa Hatta, Shizuo Akira, Yoichi Matsuda, Hirohei Yamamura, Hiroki Otani and Yasuhiro Minami: "Spatio-temporally regulated expression of receptor tyrosine kinases, mRor1, mRor2, during mouse development : implications in development and function of the nervous system."Genes to Cells. 4. 41-56 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] N.Tamura, Y.Ogawa, H.Chusho, K.Nakamura, K.Nakao, M.Suda, M.Kasahara, R.Hashimoto, G.Katsuura, M.Mukoyama, H.Itoh, Y.Saito, I.Tanaka, H.Otani, M.Katsuki and K.Nakao: "Cardiac fibrosis in mice lacking brain natriuretic peptide."Proc. Natl. Acad. Sci. USA. (in press).
Description
「研究成果報告書概要(欧文)」より
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