| Research Abstract |
The aim of this study is to identify genes which relate to susceptibility for periodontitis by using RNA arbitrarily primed PCR(RAP-PCR). RAP-PCR enables to show finger-print of a difference of mRNA expression on a gel. In first stage, human neutrophils were separated from peripheral blood of 6 healthy volunteers, stimulated with P.gingivalis-LPS and E.coli-LPS as controls. The RAP-PCR results demonstrated that differential gene expression was caused by P.gingivalis-LPS stimulation in neutrophils(Dentistry in Japan, 1999). In the second stage, the sequencing and semiquantitative RT-PCR analyses demonstrated that supervillin and vascular endothelial growth factor genes were significantly upregulated by P.gingivalis-LPS in neutrophils compared to other bacterial(A.actinomycetemcomitans, P.intermedia, E.coli)LPS and synthetic lipid A stimulations(J Periodont Res, 2001).
In the third stage, we examined differential gene expression in neutrophils from patients with generalized early-onset periodontitis patients(EOP, n=6)using RAP-PCR.Age and gender matched healthy volunteers(H, n=8)and generalized adult periodontitis patients(AP, n=6)were used as control subjects. Neutrophils separated from peripheral blood were stimulated with N-formyl-methionyl-leucyl-phenylalanine. The RAP-PCR with 45 primer pairs was performed as our previous study. The RT-PCR analyses revealed that mRNA for heat shock transcription factor 4b was significantly greater in neutrophils from EOP compared to those from the controls(H and AP). While, Kruppel-like zinc finger transcription factor 9 and muskelin mRNA were significantly lower in EOP compared to H controls(J Periodont Res, in press).
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