2000 Fiscal Year Final Research Report Summary
ANALYSIS OF CYTOPLASMIC FACTORS THAT PARTICIPATE IN GROWTH ARREST INDUCED BY OXIDATIVE STRESS
| Project/Area Number |
10470487
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | SHOWA UNIVERSITY |
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Principal Investigator |
NOSE Kiyoshi SHOWA UNIV.SCH.PHARM.SCI., PROFESSOR, 薬学部, 教授 (70012747)
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| Co-Investigator(Kenkyū-buntansha) |
MASHIMO Junn'ichi SHOWA UNIV.SCH.PHARM.SCI., RESEARCH ASSOCIATE, 薬学部, 助手 (60054045)
EGAWA Kiyoshi SHOWA UNIV.SCH.PHARM.SCI., LECTURER, 薬学部, 講師 (00095879)
SHIBANUMA Motoko SHOWA UNIV.SCH.PHARM.SCI., ASSOCIATE PROFESSOR, 薬学部, 助教授 (60245876)
NISHIYA Naoyuki SHOWA UNIV.SCH.PHARM.SCI., RESEARCH ASSOCIATE, 薬学部, 助手 (10286867)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Hic-5 / paxillin / nuclear lozalization / c-fos / focal adhesion / 接着斑 |
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| Research Abstract |
Hic-5 was isolated as a hydrogen peroxide-inducible gene that encodes a focal adhesion protein with striking similarity to paxillin. Under oxidative stress to the cell, Hic-5 was found to translocate to the nuclei, whereas paxillin did not. Truncated C-terminal parts that contain LIM domains of both proteins localized to the nuclei, and N-terminal parts were suggested to be involved in cytoplasmic retention. In particular, LD4 domain of Hic-5 was the most important for cytoplasmic localization. Cytochalasin D or phorbol ester also induced nuclear localization of Hic-5. From the survey of nuclear target genes of Hic-5, expression of c-fos was found to be inclreased in Hic-5-overexpressing cells. A luciferase reporter gene that contained 5'-regions of human c-fos gene was constructed, and introduced into fibroblasts in the absence or presence of hic-5 expression vector. When nuclear localization signal was added to Hic-5, it activated the transcription of c-fos. The DNA domain that participates in the activation localized at-1.5 kb region of c-fos gene. Hic-5 was, thus, participates both in regulation of focal adhesion signalings and in transcriptional control of specific genes.
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[Publications] Ueno, M., Sonoda, Y., Funakoshi, M., Mukaida, N., Nose, K., and Kasahara, T.: "Differential induction of JE/MCP-1 in subclones from a murine macrophage cell line, RAW 264.7 : Role of kB-3 binding protein."Cytokine. 12. 207-219 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Egawa, K., Yamori, T., Nosaka, C., Kunimoto, S., Takeuchi, T., and Nose, K.: "Deoxynubomycin is a selective anti-tumor agent inducing apoptosis and inhibiting topoisomerase I."Biol.Pharm.Bullet.. 23. 1036-1040 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishino, K., Kaneyama, S., Shibanuma, M., and Nose, K.: "Specific decrease in the level of Hic-5, a focal adhesion protein, during immortalization of mouse embryonic fibroblasts, and its association with focal adhesion kinase."J.Cell.Biochem.. 76. 411-419 (2000)
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「研究成果報告書概要(欧文)」より
