2001 Fiscal Year Final Research Report Summary
Basic research in the connection of the cause of development of symptoms of diabetes and its complication
| Project/Area Number |
10470531
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
YAMAGUCHI Tadatoshi Miyazaki Medical College, Dept of Hygiene, Ass. Prof., 医学部, 助教授 (80037598)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Hajime Miyazaki Medical College, Dept of Laboratory Medicine, Asist. Prof., 医学部, 助手 (80253839)
KASHIGE Nobuhiro Fukuoka Univ., Faculty of Pharmaceutical Sciences, Asist. Prof., 薬学部, 助手 (80185751)
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| Project Period (FY) |
1998 – 2001
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| Keywords | dihydropyrazine / DNA strand breakage / dihydrodiazepine / carbon-centered radical diabetes / diabetes / cultured cell / apoptosis |
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| Research Abstract |
Elucidation of the mechanism of DNA strand-breakage by D-glucosamine, was attempted to clear. Then we aimed at applying the enlightened results for diabetic prevention and medical treatment. It is hitherto cleared that dihydrofructosazine which was derived from D-glucosamine, has a DNA strand-breakage activity. Thus, dihydropyrazines (DHPs), similar structure to dihydrofructosazine, were synthesized and recognized to reveal the activity. Some remarkable properties of DHPs have been revealed as shown in bellows.
1. DNA strand-breakage activity
2. Generation of hydroxyl and carbon-centered radicals
3. Noble chemical reaction
4. Nucleotide sequence specificity in DNA cleavage
Further, certain new facts are beginning to become apparent. That is to say, the autoradiogram of ^<14>C-labeled DHP showed that high uptake of ^<14>C-labeled DHP was observed in the spinal cord and also the brain. The effects of DHPs on cellular viability by culture assay of several cell lines, were examined. Cell morphological changes and the induction of apoptosis-related proteins were detected.
If the presence and the production of DHPs in vitro were exhibited, the connection of DHPs with certain diseases will be elucidated in future.
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[Publications] Yamaguchi, T., Kashige, N., Matsumoto, S., Satoh, K., Yasuda, M. and Watanabe, K.: "A Carbon-Centered Radical as a Reaction Species in the DNA Strand-Breakage by D-Glucosamine"Biol. Pharm. Bull.. 21. 205-209 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamaguchi, T., Eto, M., Harano, K., Kashige, N., Watanabe, K. and Ito, S.: "New Compounds Derived from Dihydropyrazines Having DNA Strand-Breakage Activity"Tetrahedron. 55. 675-686 (1999)
Description
「研究成果報告書概要(欧文)」より
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