1999 Fiscal Year Final Research Report Summary
Evaluation of Physical Exercise from Stress Protein (2)-Evallluation of Hypoactivity and Training Effect-
| Project/Area Number |
10480004
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
体育学
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
ATOMI Yoriko University of Tokyo, the Graduate School of Arts and Sciences, Department of Life Sciences, Professor, 大学院・総合文化研究科, 教授 (90125972)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Kazunori University of Tokyo, the Graduate School of Arts and Sciences, Department of Life Sciences, Assistant Researcher, 大学院・総合文化研究科, 助手 (70251337)
HATTA Hideo University of Tokyo, the Graduate School of Arts and Sciences, Department of Life Sciences, Assistant Professor, 大学院・総合文化研究科, 助教授 (60208535)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | stress protein / running exercise / liver / skeletal muscle / HSP70 / hindlimb suspension model / muscle atrophy / GPT |
|---|
| Research Abstract |
In this study, 1) the effects of running exercise on the expression of stress protein, and 2) the effects of hindlimb suspension on the cytoskeleton tubulin/microtubule system which is the substrate for stress protein, were examined. 1) The stress protein expressions of HSP72 and HSC73, inducible and constitutive forms of HSP7Os, in exercise relating tissues (skeletal muscles and heart) and exercise non-relating tissues after two levels of exercises of low (speed : 13 m/min ; blood lactate<4 mM : LIE) and high (24 m/min ; blood lactate > 4 mM : HIE) intensities of running exercise were measured by Western blotting using anti-HSP72 and HSC73 antibodies. Serum GTP was measured as an index of liver damage after exercise. The HSP expressions after heat shock were measured as positive controls. Both the GPT levels and inducible HSP72 (3〜4 times) increased after HIE, as well as with small increases of HSC73 (20%), while only HSC73 increase was observed after LIE.Both HSP70s depressed after both exercises in hearts. 2) Tubulin content in the 5-day-suspended soleus muscles was significantly and specifically decreased, while that in the stretched muscles increased 3 times at 5 days and over 3 times at the 10 days of suspension. αB-crysallin content in precipitate fraction of muscle protein increased in suspended soleus muscles, suggesting that the association of αB-crystallin with denatured proteins in precipitate fraction increased in suspended muscle. From these results, habitual physical activity and low intensity of exercise express constitutive expressed form of HSP70 (HSC73) and higher intensity of exercise expresses inducible form of HSP70 (HSP72).
|
