| Research Abstract |
Our results are summarized as follows:
1. Regulation of cytokine signaling by PTPεC
(1) PTPεC strongly suppressed IL-6-induced M1 differentiation in PTPεC activity-dependent manner. This suppression is due to suppression of JAK-activation.
(2) This suppression by PTPεC was observed in STAT-activation through IL6R and IL10R, but not in those via IFNα/βR and IFNγR.
2. Structure and function of a novel dual specificity phosphatase, MKP-7
(1) MKP-7 has Rhodariese domain, catalytic domain, NLS, and NES.
(2) Substrate specificity is in the order ot JNK>p38>>ERK.
(3) MKP-7 is a shuttle protein between cytosol and nucleus.
3. Structure and function of a novel PP1-inhibitor, 1-4
(1) 1-4 strongly inhibits PP1, IC_<50> being 0.2 nM.
(2) 1-4interacts with PP1C on at least three sites.
4. Structure-function relationship of tautomycin and its related compounds
(1) Different moieties of tautomycin are involved in protein phosphatase inhibition and induction of apoptosis.
(2) Tautomycetin is found to be a strong and specific inhibitor for PP1.
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