2001 Fiscal Year Annual Research Report

DNA修復と組換えのクロストーク:エンドジョイニングを介する二つの機構

Research Project

Project/Area Number	10480189
Research Institution	The Kitasato Institute
Principal Investigator	池田日出男社団法人北里研究所, 基礎研究所, 部長 (10012775)
Keywords	染色体異常 / DNA二本鎖切断 / エンドジョイニング / 非相同組換え / 栄養飢餓 / 塩基のアルキル化 / DNAグリコシラーゼ / DNAトポイソメラーゼII
Research Abstract	本研究では、染色体異営の原因となるDNA二本鎖切断、エンドジョイニング、非相同組換えの起こるメカニズム及びDNA修復機構との関わりについて、大腸菌及び酵母のアッセイ系を用いて調べた。まず、大腸菌においては複製に関わるDnaBヘリケースと相同組換えに関わるExo VIII(RecE)エキソヌクレアーゼ及びRecTタンパク質が共に非相同組換えを促進することを示した。一方、二本鎖切断修復に関わるDNA結合タンパク質H-NSは非相同組換えを抑制することを示した。さらに、大腸菌を栄養飢餓の状態に置いたときにDNA二本鎖切断及び非相同組換えの亢進が起こること、一本鎖切断が塩基のアルキル化によって誘導されること、さらに、TagおよびAlkA DNAグリコシラーゼがこの組換えを抑制することを示した。従って、非相同組換えは種々の修復機構と密接に関わっていることを見出した。出芽酵母においては、DNAトポイソメラーゼII阻害剤が非相同組換えを誘導することを見出し、トポイソメラーゼの崩壊による二本鎖切断とエンドジョイニングを経由してDNAの再編成に至ると考えた。さらに、ウエルナー症候群原因遺伝子の産物WRNと会合するタンパク質WHIPを見出した。出芽酵母WHIPはWRNホモログSgs1と遺伝学的に相互作用があり、二重変異株はより早く老化することが分かった。分裂酵母のDNA複製に必須なHsk1キナーゼの温度感受性変異とrad21変異が合成致死性を示した。これらは、コヒーシンがDNA複製とも密接な関連を持つことを示した。

Research Products
(14 results)

All Other

All Publications (14 results)

[Publications] Shanado, Y, Hanada, K., Ikeda.H.: "Suppresion of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS."Mol.Gen.Genet.. 265. 242-248 (2001)
[Publications] Tatebayashi, K., Tani, T., Ikeda.H.: "Fission yeast Moglp homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)^+ RNA metabolism"Genetics.. 157. 1513-1522 (2001)
[Publications] Kang, S.-W., Ikeda H.: "WRN homolgues in non-human systems. In Werner syndrome: Implications for aging and cancer."Gann Monograph on Cancer Reserch.. 49. 137-145 (2001)
[Publications] Onda, M., Yamaguchi, J., Hanada, K., Asami, Y., Ikeda.H.: "Role of DNA ligase in the illegitimate recombination that generates λbio transducing phages"Genetics. 158. 29-39 (2001)
[Publications] Takeda, T., Ogino, K., Tatebayashi, K, Ikeda.H., Arai, K, Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast."Mol.Biol.Cell.. 12. 1257-1274 (2001)
[Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda, H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast"Nucleic Acid Res.. 29. 1326-1333 (2001)
[Publications] Shanado, Y., Hanada, K., Ikeda H: "Suppresion of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS"Mol.Gen.Genet.. 265. 242-248 (2001)
[Publications] Tatebayashi, K., Tani, T., Ikeda H: "Fission yeast Moglp homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)^+ RNA metabolism"Genetics. 157. 1513-1522 (2001)
[Publications] Kang, S.-W., Ikeda H.: "WRN homolgues in non-human systems. In Werner syndrome : Implications for aging and cancer"Gann Monograph on Cancer Reserch. 49. 137-145 (2001)
[Publications] Onda, M., Yamaguchi, J., Hanada, K., Asami, Y., Ikeda.H.: "Role of DNA ligase in the illegitimate recombination that generates λbio transducing phages"Genetics. 158. 29-39 (2001)
[Publications] Takeda, T., Ogino, K., Tatebayashi, K., Ikeda.H., Arai, K., Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast"Mol.Biol.Cell. 12. 1257-1274 (2001)
[Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda.H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast"Nucleic Acid Res.. 29. 1326-1333 (2001)
[Publications] Hanada, K., Yamashita, T., Shobuike, Y., Ikeda.H.: "Role of DnaB belicase in UV-induced illegitimate recombination in E.coli"J.Bacteriol. 183. 4964-4969 (2001)
[Publications] Kawabe.Y., Branzei, D.Hayashi, T., Suzuki.H., Masuko.T., Onda.T., Heo, S.J., Ikeda H., Shimamoto, A., Furuichi, Y., Seki, M., Enomoto, T.: "A novel protein interacts with the Werner's syndrome gone product physically and functionally"J.Biol.Chem. 276. 20364-20369 (2001)

2001 Fiscal Year Annual Research Report

DNA修復と組換えのクロストーク:エンドジョイニングを介する二つの機構

Principal Investigator

池田 日出男 社団法人北里研究所, 基礎研究所, 部長 (10012775)

Research Products

[Publications] Shanado, Y, Hanada, K., Ikeda.H.: "Suppresion of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS."Mol.Gen.Genet.. 265. 242-248 (2001)

[Publications] Tatebayashi, K., Tani, T., Ikeda.H.: "Fission yeast Moglp homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)^+ RNA metabolism"Genetics.. 157. 1513-1522 (2001)

[Publications] Kang, S.-W., Ikeda H.: "WRN homolgues in non-human systems. In Werner syndrome: Implications for aging and cancer."Gann Monograph on Cancer Reserch.. 49. 137-145 (2001)

[Publications] Onda, M., Yamaguchi, J., Hanada, K., Asami, Y., Ikeda.H.: "Role of DNA ligase in the illegitimate recombination that generates λbio transducing phages"Genetics. 158. 29-39 (2001)

[Publications] Takeda, T., Ogino, K., Tatebayashi, K, Ikeda.H., Arai, K, Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast."Mol.Biol.Cell.. 12. 1257-1274 (2001)

[Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda, H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast"Nucleic Acid Res.. 29. 1326-1333 (2001)

[Publications] Shanado, Y., Hanada, K., Ikeda H: "Suppresion of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS"Mol.Gen.Genet.. 265. 242-248 (2001)

[Publications] Tatebayashi, K., Tani, T., Ikeda H: "Fission yeast Moglp homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)^+ RNA metabolism"Genetics. 157. 1513-1522 (2001)

[Publications] Kang, S.-W., Ikeda H.: "WRN homolgues in non-human systems. In Werner syndrome : Implications for aging and cancer"Gann Monograph on Cancer Reserch. 49. 137-145 (2001)

[Publications] Onda, M., Yamaguchi, J., Hanada, K., Asami, Y., Ikeda.H.: "Role of DNA ligase in the illegitimate recombination that generates λbio transducing phages"Genetics. 158. 29-39 (2001)

[Publications] Takeda, T., Ogino, K., Tatebayashi, K., Ikeda.H., Arai, K., Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast"Mol.Biol.Cell. 12. 1257-1274 (2001)

[Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda.H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast"Nucleic Acid Res.. 29. 1326-1333 (2001)

[Publications] Hanada, K., Yamashita, T., Shobuike, Y., Ikeda.H.: "Role of DnaB belicase in UV-induced illegitimate recombination in E.coli"J.Bacteriol. 183. 4964-4969 (2001)

[Publications] Kawabe.Y., Branzei, D.Hayashi, T., Suzuki.H., Masuko.T., Onda.T., Heo, S.J., Ikeda H., Shimamoto, A., Furuichi, Y., Seki, M., Enomoto, T.: "A novel protein interacts with the Werner's syndrome gone product physically and functionally"J.Biol.Chem. 276. 20364-20369 (2001)

池田日出男社団法人北里研究所, 基礎研究所, 部長 (10012775)