Functional analysis of Wnt genes expressed in the dorsal neural tibe of the mouse embryo

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 10480208
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: TAKADA Shinji Kyoto University, Graduate School of Science, Professor, 大学院・理学研究科, 助教授 (60206753)
• Project Period (FY): 1998 – 2001
• Keywords: development / spinal cord / mouse / pattern formation / cell-cell interaction / Wnt

Research Abstract

The spinal cord is derived from the neural plate, an epithelial sheet overlying the notochord and flanked by epidermal ectoderm. The neuroepithelial cells are highly proliferative and finally differentiate into the numerous types of neurons including the motor neuron and interneurons. In the developing spinal cord, signals from the roof plate are required for the development of three classes of dorsal interneuron ; D1,D2 and D3, listed from dorsal to ventral. I demonstrate that absence of Wnt1 and Wnt3a, normally expressed in the roof plate, leads to diminished development of D1 and D2 neurons and a compensatory increase in D3 neuron populations. This occurs without significantly altered expression of BMP and related genes in the roof plate. Moreover, Wnt3a protein induces expression of D1 and D2 markers in the isolated medial region of chick neural plate. Thus, Wnt signaling plays a critical role in the specification of cell types for dorsal interneurons.

Research Products

• [Publications] Muroyama Y, Fujihara M, Ikeya M, Kondoh H, Takada S: "Wnt signaling plays an essential role in neuronal specification of the dorsal spinal cord"Genes Dev.. 16. 548-553 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ikeya, M, Takada, S.: "Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome"Development. 125. 4969-4976 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shibamoto, S., Higano, K., Takada, R., Ito, F., Takeichi, M., Takada, S.: "Cytoskeletal reorganization by soluble Wnt-3a protein signaling"Genes Cells. 3. 659-670 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohbayashi, N., Shibayama, M., Kurotaki, Y., Imanishi, M., Fujimori, T., Itoh, N., Takada, S.: "Fgf18 is required for cell proliferation and differentiation during osteogeneses and chondrogeneses"Genes Dev.. 16(印刷中). (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ikeya, M., Takada, S.: "Wnt-3a is required for somite specification along anteroposterior axis of the mouse embryo and regulates cdx-1 expression"Mech. Dev.. 103. 27-33 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamaguchi, T.P., Takada, S., Yoshikawa, Y., Wu, N., McMahon, A.P.: "T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification"Genes Dev.. 13. 3185-3190 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shibamoto S., Higano K., Takada R, Ito F., Takeichi M., Takada S.: "Cytoskeletal reorganization by soluble Wnt-3a protein signaling"Genes Cells. 3. 659-670 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ikeya M, Takada S.: "Wnt signalign from the dorsal neural tube is required for the formation of the medial dermomyotome"Development. 125. 4969-4976 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamaguchi T. P., Takada S., Yoshikawa Y., Wu N., McMahon A. P. (first two authors attributed equally to this work): "T (Brachyury) is a direct taraet of Wnt3a during paraxial mesoderm specification"Genes Dev.. 13. 3185-3190 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ikeya M., Takada S.: "Wnt-3a is required for somite specification along anteroposterior axis of the mouse embryo and regulates cdx-1 expression"Mech. Dev.. 103. 27-33 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Muroyama Y, Fujihara M, Ikeya M, Kondoh H, Takada S: "Wnt signalign plays an essential role in neuronal specification of the dorsal spinal cord"Genes Dev.. 16. 548-553 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohbayashi N., Shibayama M., Kurotaki Y., Imanishi M., Fujimori T., Itoh N., Takada S.: "Fgf18 is required for cell proliferation and differentiation during osteogenesis and chondrogenesis"Genes Dev.. 16(in press). (2002)
  • Description: 「研究成果報告書概要(欧文)」より