2000 Fiscal Year Final Research Report Summary
New methods for gene identification based on genomic analysis and their application for molecular breeding in plants.
| Project/Area Number |
10554048
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
遺伝
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
IIDA Shigeru National Institute for Basic Biology, Proffessor, 基礎生物学研究所, 教授 (30012777)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yoshikazu Institute for Fundamental Research, Suntory Ltd., Senior researcher, 基礎研究所, 主任研究員
OZEKI Yoshihiro Tokyo University of Agricultureand Technology, Associate professor, 工学部・生命工学科, 助教授 (50185592)
INAGAKI Yoshishige National Institute for Basic Biology, Research associate, 基礎生物学研究所, 助手 (50280764)
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| Project Period (FY) |
1998 – 2000
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| Keywords | anthocyanin biosynthesis gene / carnation / floricultural plants / gene identification / genome analysis / molecular breeding / the common morning glory / the Japanese morning glory |
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| Research Abstract |
The Japanese morning glory (Ipomoea nil) displaying blue flowers has an extensive history of genetic and physiological studies. A number of its spontaneous mutants related to the colors and shapes of the flowers and leaves have been isolated. Several lines of evidence indicate that an En/Spm-related transposable element Tpn1 and its relatives are major sources of these spontaneous mutations. We have developed a new method to identify a gene tagged by insertion of Tpn1 and its relatives, which we named Simplified Transposon Display (STD). Subsequently, we have successfully applied a purple-mutable allele conferring purple flowers with blue spots and sectors. The purple gene identified by STD was found to encode a vacuolar Na+/H+ exchanger that is responsible for increase vacuolar pH for blue flower coloration. We have also characterized carnation varieties and succeeded to identify new Ac/Ds-related transposable elements within the CHI and DFR senes for anthocyanin pigments biosynthesis. The integration of the transposable elements into these genes result in the altered pigmentation phenotypes. We were also able to generate new torenia varieties displaying pale flowers by introducing the CHS or DFR cDNA sequences.
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