1999 Fiscal Year Final Research Report Summary
Rapid identification of races and species-specific pathotypes using DNA-fingerprinting
| Project/Area Number |
10556011
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
植物保護
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| Research Institution | Kobe University |
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Principal Investigator |
MAYAMA Shigeyuki Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (00112251)
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| Co-Investigator(Kenkyū-buntansha) |
TOSA Yukio Kobe University, The Graduate School of Science and Technology, Associate Professor, 自然科学研究科, 助教授 (20172158)
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| Project Period (FY) |
1998 – 1999
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| Keywords | blast fungus / Magnaporthe grisea / race / DNA-fingerprinting |
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| Research Abstract |
DNA fingerprinting with miltilocus probes, MAGGY and MGR586, were conducted to investigate the population structure of the rice blast fungus, Magnaporthe grisea in Japan. Analyses of ca. 300 isolates revealed that two discrete lineages exist in the current rice blast population in Japan. They were designated as JL1 and JL2. There two lineages corresponded to two of the five lineages previously detected in collections made before 1960, suggesting that the structure of the rice blast population in Japan had drastically changed during 1960-1976. Rice blast population in Vietnam were also subjected to DNA fingerprinting with MAGGY probe and the pathotype analysis. These analyses revealed that the blast population in the northern region of Vietnam is genetically more diverse than that in the southern region. In both populations no relationships were detected between lineages and races, suggesting that the "lineage exclusion hypothesis" is not applicable to those populations.
DNA fingerprinting experiments were carried out for establishing a system of DNA-diagnosis of six host-specific subgroups of M. grisea ; Oryza isolates, Triticum isolates, Panicum isolates, Setaria isolates, Eleusine isolates, and Digitaria isolates. We propose the following formulas for distinguishing those subgroups.
(1)HindIII+MGR583 : to identify each group
(2)SalI+MAGGY : to identify the Oryza and the Setaria isolates
(3)EcoRV+MGR583 : to identify the Triticum isolates
(4)HindIII+Pot2 : to identify the Digitaria isolates
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