1999 Fiscal Year Annual Research Report

自然界に存在しないW精子の機能の検定と応用試験研究

Research Project

Project/Area Number	10556063
Research Institution	Nagoya University
Principal Investigator	島田清司名古屋大学, 大学院・生命農学研究科, 教授 (40065579)
Co-Investigator(Kenkyū-buntansha)	齋藤昇名古屋大学, 大学院・生命農学研究科, 助手 (40211924) 友金弘日本獣医畜産大学, 獣医畜産学部, 教授 (30023493)
Keywords	ニワトリ / 精子 / 卵細胞質内精子注入法 / W性染色体 / ハムスター / 受精能 / 性分化 / アロマターゼインヒビター
Research Abstract	鳥類では、雄がZZの性染色体を有しており、雌がZWの性染色体を有している。従って、精子はZ性染色体を常に有し、W性染色体を有する卵と受精すると雌が生まれ、Z性染色体を有する卵と受精すると雄が生まれるため、雌の卵子によって性が決定される。ニワトリ初期胚にアロマターゼインヒビターを投与することにより、本来雌になるニワトリが雄に性転換し、精巣を持つことを確認した。その精巣の中にはZと共にWの性染色体を有する精子が約50%存在することをFISH法を用いて確認した。そして、このW精子が受精能を有しているかを検討するために、卵細胞質内精子注入法(ICSI)を用いてハムスター卵子に性転換したニワトリの精子を注入し検討した。ICSIを用いて48個のハムスター卵子に精子を注入した結果、注入後24時間のハムスター卵子の生存率は92%であり、68%が前核形成像を示した。以上の結果から、W精子が受精能を有している可能性を示した。そして、将来Z精子からW精子が分離できW精子のみを用いて受精させれば、雌だけが生まれるように雌雄の産み分けが出来る可能性を示唆した。

Research Products
(22 results)

All Other

All Publications (22 results)

[Publications] 島田清司: "雄と雌が決まる仕組み;魚から鳥、哺乳類まで"生命誌. 7. 4-9 (1999)
[Publications] Bungo T.: "Effects of naloxone on the anorexic action by glucagon-like peptide-1(7-36) in the neonatal chick."Japanese Poultry Science. 36. 109-115 (1999)
[Publications] Furuse M.: "Central gastrin inhibits feeding behavior and food passage in neonatal chicks."Life Sciences. 65. 305-311 (1999)
[Publications] Bungo T.: "Intraventricular injection of glucagon-like peptide-1 inhibits water intake of the neonatal chick."Japanese Poultry Science. 36. 229-235 (1999)
[Publications] Saito N.: "Gene expression of arginin vasotocin in ovarian and uterine tissues of the chicken."Asian-Australasian Journal of Animal Science. 12. 695-701 (1999)
[Publications] Saito N.: "Effects of naloxone on neurohypophysial peptides release by hypertonic stimulation in chicks."General and Comparative Endocrinology. 115. 228-235 (1999)
[Publications] Bungo T.: "Influence of intracerebroventricular administration of morphine and kyotrophin on food intake of the neonatal chick."Japanese Poultry Science. 36. 319-324 (1999)
[Publications] Ieda T.: "Effect of low calcium diet on messenger ribonucleic acid levels of calbindin-D28K of intestine and shell gland in laying hens in relation to egg shell quality."Japanese Poultry Science. 36. 295-303 (1999)
[Publications] Bungo T.: "Effects of substitution of N-terminal amino acid of glucagon-like peptide-1(7-36) amide on food intake of the neonatal chick."Life Sciences. 25. PL295-PL299 (1999)
[Publications] Bungo T.: "Intracerebroventricularly administration of glucagon-like peptide-1 induces sleep-like behavior in the neonatal chick."Japanese Poultry Science. 36. 377-381 (1999)
[Publications] Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing hormone receptor, P450 17α-hydroxylase and P450 aromatase in isolated grnulosa, theca interna and theca externa layers of chicken ovarian follicles during follicular growth"Animal Science Journal. 70. 12-17 (1999)
[Publications] Bungo T.: "Effects of substitution of N-terminal amino acid of glucagon-like peptide-1(7-36) amide on food intake of the neonatal chick."Life Sciences. 25. PL295-PL299 (1999)
[Publications] Tan F.-I.: "Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken."Biology of Reproduction. 62. 8-15 (2000)
[Publications] Nishikimi H.: "Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken."Molecular Reproduction and Development. 55. 20-30 (2000)
[Publications] Suzuki T.: "Cytogenetical assignment of 29 functional genes on chicken microchromosomes by FISH."Cytogenetics Cell Genet.. (in press). (2000)
[Publications] Suzuki T.: "Comparative FISH mapping on Z chromosomes of chicken and Japanese quail"Cytogenetics Cell Genet.. 87. 22-26 (1999)
[Publications] Sasaki T.: "Regulation of opioid peptides on the release of arginine vasotocin in the hen."Journal of Experimental Zoology. (in press). (2000)
[Publications] Ieda T.: "Changes in parathyroid hormone related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle."General and Comparative Endocrinology. (in press). (2000)
[Publications] Suzuki T.: "Cytogenetical mapping of thirty-one functional genes on chicken chromosomes by the direct R-banding FISH."Cytogenetics Cell Genet.. 87. 32-40 (1999)
[Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)
[Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)
[Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

1999 Fiscal Year Annual Research Report

自然界に存在しないW精子の機能の検定と応用試験研究

Principal Investigator

島田 清司 名古屋大学, 大学院・生命農学研究科, 教授 (40065579)

Research Products

[Publications] 島田 清司: "雄と雌が決まる仕組み;魚から鳥、哺乳類まで"生命誌. 7. 4-9 (1999)

[Publications] Bungo T.: "Effects of naloxone on the anorexic action by glucagon-like peptide-1(7-36) in the neonatal chick."Japanese Poultry Science. 36. 109-115 (1999)

[Publications] Furuse M.: "Central gastrin inhibits feeding behavior and food passage in neonatal chicks."Life Sciences. 65. 305-311 (1999)

[Publications] Bungo T.: "Intraventricular injection of glucagon-like peptide-1 inhibits water intake of the neonatal chick."Japanese Poultry Science. 36. 229-235 (1999)

[Publications] Saito N.: "Gene expression of arginin vasotocin in ovarian and uterine tissues of the chicken."Asian-Australasian Journal of Animal Science. 12. 695-701 (1999)

[Publications] Saito N.: "Effects of naloxone on neurohypophysial peptides release by hypertonic stimulation in chicks."General and Comparative Endocrinology. 115. 228-235 (1999)

[Publications] Bungo T.: "Influence of intracerebroventricular administration of morphine and kyotrophin on food intake of the neonatal chick."Japanese Poultry Science. 36. 319-324 (1999)

[Publications] Ieda T.: "Effect of low calcium diet on messenger ribonucleic acid levels of calbindin-D28K of intestine and shell gland in laying hens in relation to egg shell quality."Japanese Poultry Science. 36. 295-303 (1999)

[Publications] Bungo T.: "Effects of substitution of N-terminal amino acid of glucagon-like peptide-1(7-36) amide on food intake of the neonatal chick."Life Sciences. 25. PL295-PL299 (1999)

[Publications] Bungo T.: "Intracerebroventricularly administration of glucagon-like peptide-1 induces sleep-like behavior in the neonatal chick."Japanese Poultry Science. 36. 377-381 (1999)

[Publications] Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing hormone receptor, P450 17α-hydroxylase and P450 aromatase in isolated grnulosa, theca interna and theca externa layers of chicken ovarian follicles during follicular growth"Animal Science Journal. 70. 12-17 (1999)

[Publications] Bungo T.: "Effects of substitution of N-terminal amino acid of glucagon-like peptide-1(7-36) amide on food intake of the neonatal chick."Life Sciences. 25. PL295-PL299 (1999)

[Publications] Tan F.-I.: "Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken."Biology of Reproduction. 62. 8-15 (2000)

[Publications] Nishikimi H.: "Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken."Molecular Reproduction and Development. 55. 20-30 (2000)

[Publications] Suzuki T.: "Cytogenetical assignment of 29 functional genes on chicken microchromosomes by FISH."Cytogenetics Cell Genet.. (in press). (2000)

[Publications] Suzuki T.: "Comparative FISH mapping on Z chromosomes of chicken and Japanese quail"Cytogenetics Cell Genet.. 87. 22-26 (1999)

[Publications] Sasaki T.: "Regulation of opioid peptides on the release of arginine vasotocin in the hen."Journal of Experimental Zoology. (in press). (2000)

[Publications] Ieda T.: "Changes in parathyroid hormone related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle."General and Comparative Endocrinology. (in press). (2000)

[Publications] Suzuki T.: "Cytogenetical mapping of thirty-one functional genes on chicken chromosomes by the direct R-banding FISH."Cytogenetics Cell Genet.. 87. 32-40 (1999)

[Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

[Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

[Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

島田清司名古屋大学, 大学院・生命農学研究科, 教授 (40065579)

[Publications] 島田清司: "雄と雌が決まる仕組み;魚から鳥、哺乳類まで"生命誌. 7. 4-9 (1999)