1999 Fiscal Year Final Research Report Summary
Polymorphism's of mitochondrial DNA and selection of Japanese Black
| Project/Area Number |
10556064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied animal science
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| Research Institution | Kobe University |
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Principal Investigator |
TSUJI Soichi KOBE UNIVERSITY, FACULTY OF AGRICULTURE, PROPFESSOR, 農学部, 教授 (10031220)
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| Co-Investigator(Kenkyū-buntansha) |
MANNEN Hideyuki KOBE UNIVERSITY, FACULTY OF AGRICULTURE, ASSISTANT PROPFESSOR, 農学部, 助手 (20263395)
MUKAI Fumio KOBE UNIVERSITY, FACULTY OF AGRICULTURE, PROPFESSOR, 農学部, 教授 (50093323)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Japanese Black / mitochondria D-loop / maternal inheritance / PCR-SSCP / marbling / rib eye area |
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| Research Abstract |
Japanese black is only one domestic animal which produce economically competitive beef with foreign products, because of producing high grade beef. The genes by which the high quality beef being produced are not yet known. Almost of all researches are concentrated their study on the genome DNA from the nuclei, however, we had paid attention on the genome from mitochondria for past few years. Japanese Black are classified into five groups depending on D-loop DNA sequence of mitochondria. We found that a group, type 4, produce high quality beef. 1)To ascertain if this mitochondrial effect is observed in commercial reproductive herd, cows of more than 8,000 were classified into 5 groups by mitochondrial DNA type. The effect of mitochondrial type is not clear because of environmental effects, such as feeding condition, year season, feedlot etc. But in Hyogo prefecture, the mitochondrial effect was only observed in steers of 28month old finishing. If D-loop has some physiological effects on beef production, as the D-loop region does not code any protein, so that mutations other region than D-loop region, which coordinates with D-loop type should be present. We selected two animals from each mitochondrial type and DNA sequence of whole mitochondria genome was determined. We found type 4 specific mutation in 16SrRNA which may affect on protein synthesis in mitochondria.
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