2000 Fiscal Year Final Research Report Summary
Analysis of regulation of host defense response by osteopopontin and its applocation to diagnosis and therapy strategy
| Project/Area Number |
10557024
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Experimental pathology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
UEDE Toshimitsu Hokkaido Univ., Inst.Genet.Med., Prof., 遺伝子病制御研究所, 教授 (00160185)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Masahiro Meneki Seibutsu Co.Ltd., Chief., 次長
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| Project Period (FY) |
1998 – 2000
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| Keywords | osteopontin / cancer / prognosis / atherosclerosis |
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| Research Abstract |
1. Highly metastatic adenocarcinoma cells tend to secrete osteopontin(OPN). OPN binds to β1 integrin-containing receptors in an autocrine fashion and induce motility of tumor cells. The association of β1 integrins with CD44v on tumor cell surface is essential for OPN-induced tumor cell motility.
2. The new-vessel formation as defined by CD34 positive endothels was significantly increased in OPN and VEGF positive stage I lung adenocarcinoma tissues and corelated very well with poor prognosis of patients.
3. Upon high fat diet feeding, OPN transgenic mice developed considerably severe atherosclerosis as compared to control mice. In OPN transgenic mice, macrophages within atherosclerotic lesions expressed significant levels of OPN.
4. Upon intravenous injection of zymosan, mice develoved liver granuloma, the development of granuloma was significantly inhibited by simultaneous injection of anti-OPN antibody, indicating the critical role of OPN in the granuloma development.
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[Publications] S.Kon, M.Maeda, T.Segawa, Y.Hagiwara, Y.Horikoshi, S.Chikuma, K.Tanaka, MM.Rashid, M.Inobe, AF.Chambers, T.Uede: "Antibodies to different peptides in osteopontin reveal complexities in the various secreted forms."J.Cell.Biochem.. 77(3). 487-498 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] R.Kawashima, S.Mochida, A.Matsui, Y.YouLuTuz, K.Ishikawa, K.Toshima, F.Yamanobe, M.Inao, H.Ikeda, A.Ohno, S.Nagoshi, K.Fujiwara: "Expression of osteopontin in kupffer cells and hepatic macrophages and Stellate cells in rat liver after carbon tetrachloride intoxication : a possible factor for macrophage migration into hepatic necrotic areas."Biochem.Biophys.Res.Commun.. 256(3). 527-531 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] YU.Katagiri, J.Sleeman, H.Fujii, P.Herrich, H.Hotta, K.Tanaka, S.Chikuma, H.Yagita, K.Okumura, M.Murakami, I.Saiki, AF.Chambers, T.Uede: "CD44 variants but not CD44s cooperate with β1-containing integrins to permit cells to bind to osteopontin independently of arginine-glycine-aspartic acid, thereby stimulating cell motility and chemotaxis."Cancer Res.. 59(1). 219-226 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] J.Iizuka, Y.Katagiri, N.Tada, M.Murakami, T.Ikeda, M.Sato, K.Hirokawa, S.Okada, M.Hatano, T.Tokuhisa, T.Uede: "Introduction of an osteopontin gene confers the increase in B1 cell population and the production of anti-DNA autoantibodies."Lab.Invest.. 78(12). 1523-1533 (1998)
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「研究成果報告書概要(欧文)」より
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