1999 Fiscal Year Final Research Report Summary
Preparation of peptide vaccines according to the cassette theory against newly emerged influenza virus
| Project/Area Number |
10557025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Experimental pathology
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| Research Institution | SHIGA UNIVERSITY OF MEDICAL SCIENCE (1999)
Hokkaido University (1998) |
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Principal Investigator |
OGASAWARA Kazumasa SHIGA UNIVERSITY OF MEDICAL SCIENCE, DEPARTMENT OF 2nd PATHOLOGY, PROFESSOR, 医学部, 教授 (20169163)
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| Co-Investigator(Kenkyū-buntansha) |
KIDA Hiroshi HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF VETERINARY MEDICINE, PROFESSOR, 大学院・獣医学研究科, 教授 (10109506)
ONOE Kazunori HOKKAIDO UNIVERSITY, INSTITUTE OF IMMUNOLOGICAL SCIENCE, PROFESSOR, 免疫科学研究所, 教授 (40002117)
HAYASE Yoneko SHIGA UNIVERSITY OF MEDICAL SCIENCE, DEPARTMENT OF 2nd PATHOLOGY, RESEARCH ASSOCIATE, 医学部, 助手 (40228606)
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| Project Period (FY) |
1998 – 1999
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| Keywords | MHC / peptide vaccine / newly emerged influenza virus / HA / cassette theory / CTL / NP / anti-CD40 antibody |
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| Research Abstract |
1. According to the cassette theory, we prepared a synthetic peptide vaccine against H5 subtype influenza viruses. The peptide, however, could not induce neutralizing antibodies specific for H5 subtype influenza viruses in mice. This result suggests that all antibodies produced by immunizing with the peptides prepared according to the cassette theory could not recognize their original proteins. Thus, we are preparing a new peptide which can mimic the original protein. Namely, it contains a circular peptide formed with a disulfied bond and an I-AィイD1bィエD1 binding component (46F/54A).
2. After inoculation with NP366-374 peptide of a H3 subtype influenza virus in multilamellar liposome and anti-CD40 monoclonal antibody into nasal cavity of mice, the H3 subtype influenza virus was challenged. The virus infection could be prevented for 2 months by CTL activated in mice that were inoculated with both materials. It seems that the NP peptide vaccine is effective in preventing other subtypes influenza viruses, because NP contains little mutation. Thus, we are protecting infection of H5 subtype influenza viruses using liposomal NP366-374 peptide and anti-CD40 monoclonal antibody.
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[Publications] Matsui, N, Ogasawara, K., Takami, K., Namba, K., Takahashi, A., Fukui, Y., Sasazuki, T., Iwabuchi K., Good, R.A. and Onoe, K.: "Prevention of infection of influenza virus in DQ6 mice, a human model, by a peptide vaccine prepared according to the cassette theory"Vaccines. 17. 1161-1168 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Takahashi, A., Iwabuchi K., Suzuki, M., Ogasawara, K., Nishihira, J., and Onoe, K.: "Antisense macrophage migration inhibitory factor (MIF) prevents anti-IgM mediated growth arrest and apoptosis of a murine B cell line by regulating cell cycle progression"Microbiol. Immunol.. 43. 61-67 (1999)
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[Publications] Kariyone, A., Yamamoto, S., Nagasaka-Kametaka, A., Harada, M., Takahashi, A., Ogasawara, K., and Takatsu, K.: "Amino acid residues of T cell epitope of the α antigen of Mycobacteria critical for Vβ11ィイD1+ィエD1Th1-cells"Infect. Immun.. 67. 4312-4319 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Itoh, D., Ogasawara, K., Matsushita, K., Morohashi, T., Namba, K., Matsuki, N., Kitaichi, N., Inuyama, Y., Hosokawa, M., Nakayama, E., Iwabuchi, K., and Onoe, K.: "Effective priming of cytotoxic T lymphocyte precursors by subcutaneous administration of peptide antigen in liposomes accompanied with anti-CD40 and anti-CTLA-4-antibodies"Immunobiol.. (in press).
Description
「研究成果報告書概要(欧文)」より
