• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Organ Transplantation from non-heart beating

Research Project

Project/Area Number 10557112
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field General surgery
Research InstitutionKobe University

Principal Investigator

KURODA Yoshikazu  Kobe University school of Medicine, Professor, 医学部, 教授 (70178143)

Co-Investigator(Kenkyū-buntansha) SUZUKI Yasuyuki  Kobe University school of Medicine, Research Associate, 医学部, 助手 (40304092)
KU Yonson  Kobe University school of Medicine, Professor, 医学部, 助教授 (40195615)
Project Period (FY) 1998 – 2000
KeywordsPancreas transplantation / Warm ischemic injury / Two-layer method / UW simple cold storage / Protein synthesis / Stress protein
Research Abstract

1. Based on our previous canine experiments, pancreas grafts subjected to 90 min warm ischemia, which cannot survive after implantation with no treatment, could be successfully transplanted after 5-hour preservation by the two-layer method. On the hypothesis that protein synthesis during the two-layer preservation would be associated with this graft resuscitation, the following experiments were undertaken. We preserved canine pancreases damaged by 90-min warm ischemia using the two-layer method (perfluorochemical/UW solution) for 5 hrs at 20℃. Intragraft DNA, RNA and protein synthesis were quantitated durins preservation by determining incorporation of tritiated thymidine, tritiated uridine and tritiated leucine, respctively, added in this preservation solution. Graft viability was judged from graft survival after autotransplantation in the cervical subcutaneous cavity. None of 7 grafts (0%) survived when they were immediately transplanted after warm ischemia, while all of 7 grafts (100%) transplanted after the two-layer preservation for 5 hrs at 20℃ survived. Significant increases in RNA and subsequent protein syntheses were observed during preservation by the two-layer method, not by UW simple cold storage. In contrast, DNA synthesis did not follow RNA and protein synthesis. We concluded that protein synthesis might be associated with the process of postischemic cellular recovery of the pancreas graft during mild hypothermic preservation by the two-layer method.
2. Some reports have indicated that heat shock proteins (HSPs) have important functions in cellular recovery. We studied the role of HSPs in reducing ischemia-reperfusion injury during preservation by the two-layer method. From the results of immunohistochemical examinations and western-blotting of HSPs, HSP-60 was likely associated with functional recovery of warm ischemically damaged pancreas by the two-layer method.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shinichi Matsumoto: "Evidence of protein synthesis during resuscitation of ischemically damaged canine pancreas by the two layer method"Pancreas. 20(4). 411-414 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ippei Matsumoto: "Superiority of mild hypothermic (20degrees C) preservation for pancreatic microvasculature using two-layer storage method"Pancreas. 21(3). 305-309 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi Matsumoto: "clinical application of the two-layer (University of Wisconsin solution (UW)/perfluorochemical (PFC)) method of pancreas preservation before transplantation"Transplantation. 170. 771-774 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhiro Fujino: "Possible role of heat shock protein 60 reducing ischemia-reperfusion injury in canine pancreas grafts after preservation in canine pancreas grafts after preservation by the two-layer method"Pancreas. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤野泰宏: "二層単純浸漬保存法による膵虚血再灌流障害の軽減効果"今日の移植. 12(6). 600-603 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷岡康喜: "臓器保存とviability判定法"低温医学. 25(1). 30-34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤野泰宏: "消化器外科ケアマニュアル"照林社. 3 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Deai T, Tanioka Y, Suzuki Y, Kuroda Y: "The effect of the two-layer cold storage method on islet isolation from ischemically damaged pancreas"Kobe J Med Sci. 45(3-4). 191-199 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto S, Sutherland DER, Hassoun AA, Kandaswamy R, Hiraoka K, Sageshima J, Shibata S, Alizona L, Tanioka Y, Kuoda Y: "Clinical application of the two-layer (University of Wisconsin solution (UW)/perfluorochemical (PFC)) method of pancreas preservation before transplantation"Transplantation. 170. 771-774 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto S, Fujino Y, Suzuki Y, Tanioka Y, Muramatsu S, Sugimoto T, Ku Y, Yasunami Y, Kuroda Y: "Evidence of protein synthesis during resuscitation of ischemically damaged canine pancreas by the two-layer method"Pancreas. 20(4). 411-414 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto I, Suzuki Y, Fujino Y, Tanioka Y, Deai T, Iwanaga Y, Mitsutsuji M, Iwasaki T, Tominaga M, Ku Y, Kuroda Y: "Superiority of mild hypothermic (20℃) preservation for pancreatic microvasculature using two-layer storage method"Pancreas. 21(3). 305-309 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujino Y, Suzuki Y, Tsujimura T, Takahashi T, Tanioka Y, Tominaga M, Ku Y, Kuroda Y: "Possible role of heat shock protein 60 reducing ischemic-reperfusion injury in canine pancreas grafts after preservation by the two-layer method"Pancreas. in press.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi