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2000 Fiscal Year Final Research Report Summary

Identification of genes associated with epithelial growth using Ileojejunal transposition model

Research Project

Project/Area Number 10557118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Digestive surgery
Research InstitutionTohoku University

Principal Investigator

FUKUSHIMA Kouhei  Tohoku University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20271900)

Co-Investigator(Kenkyū-buntansha) SASAKI Iwao  Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (60125557)
NAITO Hiroo  Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (90180223)
FUNAYAMA Yuji  Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (50192315)
Project Period (FY) 1998 – 2000
KeywordsShort boroel Syndrome / total colectomy / molecular biology / epithelial cell / Cell pretiferation
Research Abstract

(1)Epithelial gene screening by differential display
a.Ileojeiunal transposition (IJT) model
Ileojejunal transposition was performed using SD rats and samall intestinal epithelial cells were isolated. We subcloned 8 gene and sequenced, resulting that all these clones encoded mitochondrial genes. Differential expresssion was not confirmed, indicating that selection of mitochondrial genes may be due to technical issues accompanied with differential display.
b.Short bowel model
Shor bowel model was established in SD rats and samall intestinal epithelial cells were isolated. We subcloned 11 genes including mitochondrial genes and solute carrier family 9, isoform 3 regulator 1from 80 differential displays.
(2)ornithine decarboxylase(ODC) induction in vitro
IEC6 cells were stimulated 0, 25, 50, and 100 ng/ml glucagon-like peptide 2 (GLP2) and expression of ODC mRNA was examined. Expression of ODC mRNA was stabe after stimulation. In contast, epidermal growth factor, used as a positive control, induced ODC mRNA.
(3)Intestinal adaptation following total colectomy
Rat total volectomy model was established and gene expression of remnant ileal epithelia was analyzed. 11b-hydroxysteroi dehydrogenase type2 (11b-HSD2) was remarkablly induced in the distal ileum probablly due to enhanced aldosterone in blood.
(4)Conclusion
Difficulties in this project was due to 1.inconsistent results from ileojejunal transposition model and 2.limitation of gene screening technique, differential display.

  • Research Products

    (6 results)

All 2000 1999

All Journal Article (6 results)

  • [Journal Article] Urocorin and Corticotropin-relasing factor receptor expression in the human colonic mucosa.2000

    • Author(s)
      村松康成
    • Journal Title

      Peptides 21

      Pages: 1799-1809

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Urocortin and corticotropin-releasing factor receptor expression in the human colonic mucosa2000

    • Author(s)
      Y.Muramatsu, K.Fukushima, et al.
    • Journal Title

      Peptides 21

      Pages: 1799-1809

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of minerale corticoid receptor by sodium butyrate in small intespinal(IEC6) and colonic(T84) epithelial cell lines.1999

    • Author(s)
      福島浩平
    • Journal Title

      Digestive Diseases and Sciences 44

      Pages: 1571-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Gene induction of 11β-hydroxy steroid typeII(11β-HSD2) is essential for enhancednent of aldosttrone-induced sodium absorption1999

    • Author(s)
      佐藤 俊
    • Journal Title

      Gastroenterology 116

      Pages: A-1349

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Induction of mineralocorticoid receptor by sodium butyrate in small intestinal (IEC6) and colonic (T84) epithelial cell lines1999

    • Author(s)
      K.Fukushima et al.
    • Journal Title

      Digestive Diseases and Sciences 44

      Pages: 1571-1578

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gene induction of 11β-hydroxysteroid dehydrogenase type II (^<41>β-HSD2) is essential for enhancement of aldosterone-mediated sodium absorption after total proctocolectomy in rats.1999

    • Author(s)
      S.Sato, K.Fukushima et al.
    • Journal Title

      Gastroenterology 116

      Pages: A1349

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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