2001 Fiscal Year Final Research Report Summary
Study for development and practical use of S-DEX as an anti-adheren agent against cancer cells for peritoneal carcinomatosis
| Project/Area Number |
10557121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HAGIWARA Akeo Kyoto Prefectural University of Medicine, Medical School, Associate Professor, 医学部, 助教授 (90198648)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKURA Chouhei Kyoto Prefectural University of Medicine, Medical School, Assistant Professor, 医学部, 助手 (10285257)
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| Project Period (FY) |
1998 – 2001
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| Keywords | peritoneal carcinomatosis / dextran sulfate / adherence of cells / peritoneal metastasis of cancer / cell-adherence-molecules / cancer cells / animal experiments / anti-cancer drugs |
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| Research Abstract |
(1) The derivatives from acid mucopolysaccaride S-DEX was studies for its effects of prevention to peritoneal metastasis and problems in clinical application.
(A) Body weight change, toxic symptoms LDx values, histo-pathological findings were studied in mice. Body weight change recovered within 1 week after administration. Toxic symptoms were slight. The LD10, 50, and 90 values were 0.21, 0.34, 0.53 mg/kg. Histologically hemorrhagic inflammation, seen in colon mucosa, was improved at the end of the observation period.
(B) S-DEX, at 0.01 mg/kg, induced no abnormal values in blood cells count and biochemical analysis of blood in rabbits.
(C) There were no adverse effects on anastomosis of intestines in rats, when the anasotomosis was covered by surgical glue.
(2) The Effects of S-DEX were studied for the expression of genes and proteins with B- melanoma and gastric cacer cell lines.
(A) Using cDNA micro-tips, expression of mRNA was analyzed. P- and DCK4 were increased and Integrin 1 and ICM-2 were decreasedwith time.
(B) Western blot showed that p-15 was increased and Integrin 1 was decreased with time. Thus, analyses in mRNA nad protein evels revealed that S-DEX brakes cell-cycle and prevents cell-adherence These findings suggests that S-DEX will be safely used as anti-adherent agent against cancer cells.
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