| Project/Area Number |
10557146
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Gunma University |
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Principal Investigator |
IBUKI Yoshito Gunma Univ. Sch. Med. Dept. OB/GYN., Prof., 医学部, 教授 (40008256)
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| Co-Investigator(Kenkyū-buntansha) |
ANDOH Kazumichi Gunma Univ. Sch. Med. Dept. OB/GYN., Assistant Professor, 医学部, 講師 (20232093)
MIZUNUMA Hideki Gunma Univ. Sch. Med. Dept. OB/GYN., Associate Professor, 医学部, 助教授 (10125875)
ABE Yumiko Gunma Univ. Sch. Med. Dept. OB/GYN., Research Associate, 医学部, 助手 (70261857)
MASHIMO Ikuo Teijin SRL Labotatory, INC. Director of the board, General Manager Clinical Chemistry Lab, 研究検査部, 担当次長
HASEGAWA Yoshihisa Kitasato Univ. Sch. Vet. Med. Anim. Sci., Prof., 獣医畜産学部, 教授 (40092001)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Inhibin / Assay / Clinical Applications |
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| Research Abstract |
The anti-inhibin polyclonal antibody used in our RIA reacts with inhibin A, inhibin B, and inhibin α-monomer. Our inhibin RIA system estimates the amount of inhibin B more accurately than Monash RIA and the pattern of IR-inhibin during normal cycle reflects both inhibin A and inhibin B. We improved the sensitivity of this assay system by changing the buffer and iodination methods and obtained the minimum detection level with 0.1 IU/ml. Using this assay system, IR-inhibin levels in various clinical samples were measured. Serum IR-inhibin levels of patients with granulosa cells reflect the clinical conditions. IR-inhibin levels dropped below the detectable limit after total removal of the tumor in these patients and increased earlier in the case of relapse than other tumor markers. IR-inhibin increased earlier than inhibin A or inhibin B in the patient who manifested OHSS among women undergoing ovulation induction by ganadotropins. Therefore there is the possibility that IR-inhibin is a good marker of hyperstimulation of the ovaries. IR-inhibin was below the sensitivity level among women over 60 years old. The study of a large number of samples from women over 60 years old is ongoing, in order to determine whether IR-inhibin is suitable as a tumor marker or not.
We also developed inhibin A immunofluorometric assay and the study of inhibin A during pregnancy measured by this assay is continuing. The anti inhibin B antibody which was developed was not suitable for immunofluorometric assay.
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