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2000 Fiscal Year Final Research Report Summary

Development of antisense therapy for periodontal diseases targeted bacterial cell-division related genes

Research Project

Project/Area Number 10557199
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 矯正・小児・社会系歯学
Research InstitutionKyushu Dental College

Principal Investigator

TAKEHARA Tadamichi  Kyushu Dental College, Professor., 歯学部, 教授 (00038879)

Co-Investigator(Kenkyū-buntansha) WACHI Masaaki  Tokyo Institute of Technology, Associate Prof., 生命理工学部, 助教授 (90192822)
AKIFUSA Sumio  Kyushu Dental College, Assistant Prof., 歯学部, 助手 (40295861)
ANSAI Toshihiro  Kyushu Dental College, Associate Prof., 歯学部, 助教授 (80244789)
YAMAGISHI Jyun-ichi  Dainippon Pharmaceutical Company., 主任研究員
Project Period (FY) 1998 – 2000
KeywordsPeptidoglycan / Peridontal diseases / P.gingivalis / Cell division
Research Abstract

Peptidoglycan is known to be a heteropolymer with a unique chemical structure and biological activity, and be essential for cell viability of bacteria. We have already isolated and sequenced a gene encoding the MurC protein from Porphyromonas gingivalis (PgMurC gene), an oral anaerobic rod-shaped bacterium implicated in progressiove periodontal disease. The MurC protein functions in peptidoglycan synthesis and catalyzes the first step in the biosynthesis of cell wall peptidoglycan. The region including PgMurC gene appeared to be highly similar with mra region in E.coli, which contains genes concerned with peptidoglycan synthesis, and have been shown to be tightly clustered forming an operon. Then, we have sequenced the neibouring region of PgMurC gene, and we found that the three ORFs had a significant similarity with FtsQ (16%), FtsA (33%), and FtsZ (54%) in E.coli, respectively. The predicted FtsA from P.gingivalis (PgFtsA) had five motifs for ATPase domain, belonging to the actin fa … More mily, as in the FtsA from E.coli. When PgFtsA was overexpressed in E.coli, cell division was inhibited and its morphology changed to long filamentous cells. Electron micrographs of these cells revealed that the formation of aggregated structures existed in the E.coli cytoplasm. On the other hand, the FtsZ from P.gingivalis (PgFtsZ) possessed the clear motifs for GTP binding and hydrolysis, and the purified PgFtsZ protein exhibited GTPase activity with the following propeties different from other known FtsZ proteins ; 1) Na^+ and K^+ ions inhibited its GTPase activity. 2) PgFtsZ exhibited its GTPase activity even without Mg^<2+>, and completely retained its activity with EDTA.Very recently, a series of mutants deleted from the C-teminus of PgFtsZ were generated, and the change of their morphology were observed. We found that the delta C-177 mutant, deleted 177 amino acid residues from C-terminus, changed to the normal cells. These results suggest that amino acid residues from T281 to E330 may be important for the functional role in PgFtsZ.In order to identify amino acid residues in the corresponding region, mutant PgFtsZ proteins are currently generated through the use of site-directed mutagenesis. Less

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ansai,T. et al.: "A mur C gene in Porphyromonas gingivalis 381."Microbiology. 141. 2047-2052 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ansai,T. et al.: "Interactions between a minimal protein serine/threonine phospahatase and phosphopeptide substrate sequence."J.Biol.Chem.. 271. 24401-24407 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murata,T.,Ansai,T. et al.: "Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro"Oral Diseases. 3. 106-112 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ansai,T. et al.: "Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia."FEBS Lett.. 428. 157-160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen,X.,Ansai,T. et al.: "Isolation,cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from Prevotella intermedia."J.Bacteriol.. 181. 7107-7114 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Awano,S.,Ansai,T. et al.: "Sequencing,expression and biochemical characterization of the Porphyromonasgingivalis perO gene encoding a protein homologous to..."FEBS Lett.. 460. 139-144 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wachi,M. et al.: "Cloning,sequencing,and characterization of the ftsZ gene from Coryneform bacteria."Biochem.Biophys.Res.Commun.. 236. 383-388 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wachi,M. et al.: "A murC gene from coryneform bacteria."Appl.Microbiol.Biotechnol.. 51(2). 223-238 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Malathi,KC.,Wachi,M. et al.: "Isolation of the murI gene form Brevibactrium lactofermentum ATCC 13869 encoding D-glutamate racemase."FEMS Microbiol.Lett.. 175(2). 193-196 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ansai,T.and Takehara,T.: "Recent Research Developments in Microbiology"Research Signpost(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安細敏弘(分担執筆): "歯周病最前線-オーラルケアが守る長寿社会のQOL-"日本歯科評論. 354 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ansai, T.et al.: "A murC gene in Porphyromonas gingivalis 381."Microbiology. 141. 2047-2052 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ansai, T.et al.: "Interactions between a minimal protein serine/threonine phospahatase and phosphopeptide substrate sequence."J.Biol.Chem.. 271. 24401-24407 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wachi, M.et al.: "Cloning, sequencing, and characterization of the ftsZ gene from Coryneform bacteria."Biochem. Biophys.Res.Commun.. 236. 383-388 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, T., Ansai, T.et al.: "Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro."Oral Diseases. 3. 106-112 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ansai, T.et al.: "Purification and characterization of alkaline phosphatase containing phospho-tyrosyl phosphatase activity from the bacterium Prevotella intermedia."FEBS Lett.. 428. 157-160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wachi, M.et al.: "A murC gene from coryneform bacteria."Appl.Microbiol.Biotechnol.. 51 (2). 223-228 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Malathi, KC., Wachi, M.et al.: "Isolation of the murI gene from Brevibacterium lactofermentum ATCC 13869 encoding D-glutamate racemase."FEMS Microbiol.Lett.. 175 (2). 193-196 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chen, X., Ansai, T.et al.: "Isolation, cloning, and expression of an acid phosphatase containing phospho-tyrosyl phosphatase activity from Prevotella intermedia."J.Bacteriol.. 181. 7107-7114 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Awano, S., Ansai, T.et al.: "Sequencing, expression and biochemical characterization of the Porphyromonasgingivalis pepO gene encoding a protein homologous to human endothelin-converting enzyme."FEBS Lett.. 460. 139-144 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ansai, T.and Takehara, T.: "Phosphatases containing phosphotyrosyl phosphatase activity from Prevotella intermedia : structure, function, and evolution. (eds. by Stormer, FC., Montville, TJ.and Levin, RJ.)"Recent Research Developments in Microbiology. 4. 569-584 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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