2001 Fiscal Year Final Research Report Summary
Development of Fluorescence Microscopy for Visualizing Single Biomolecules in Living Cells
| Project/Area Number |
10558110
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biophysics
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| Research Institution | Waseda University |
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Principal Investigator |
FUNATSU Takashi Waseda University, School of Science and Engineering, Associate Professor, 理工学部, 助教授 (00190124)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Single molecule imaging / Fluorescence microscopy / mRNA / Chaperonin |
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| Research Abstract |
Single fluorescent molecules in aqueous solution were imaged for the first time at video-rate using Nipkow disk-type confocal microscopy. Performance of this method was evaluated by imaging single kinesin molecules labeled with fluorescent dyes of tetramethylrhodamine (TMR) or IC5. Photodecomposition lifetimes of the fluorophores were 〜10 sec for TMR and 〜2 sec for IC5 under the incident laser power of 0.5 W/mm^2. Both the fluorescence intensity and the photobleaching rate were proportional to the laser power from 0.65 to 3 W/mm^2. 2D sliding movement of single kinesin molecules along microtubules on glass surface, and 3D Brownian motion of individual kinesin molecules in viscous solution could be observed using this microscopy. These results indicated that this method could be applicable to the study of single molecular events in living cells at real time.
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