1999 Fiscal Year Final Research Report Summary
Development of Atomic Force Microscope System Combined with Confocal Laser Scanning Microscope to Simultaneously Measure Mechanical Stiffness and Observe Microstructure of Cultured Cells
| Project/Area Number |
10558126
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
SATO Masaaki Department of Mechatronics and Precision Engineering, Tohoku University, Professor, 大学院・工学研究科, 教授 (30111371)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Yoshiaki Product Development Department, Olympus Optical Co., Ltd., Research Manager, 光学機器開発部, 課長(研究職)
OHASHI Toshiro Department of Mechatronics and Precision Engineering, Tohoku University, Research Assistant, 大学院・工学研究科, 助手 (30270812)
MATSUMOTO Takeo Department of Mechatronics and Precision Engineering, Tohoku University, Associate Professor, 大学院・工学研究科, 助教授 (30209639)
KATAOKA Noriyuki Department of Medical Engineering, Kawasaki College of Allied Health Professions, Assistant Professor, 医用電子技術科, 講師 (20250681)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Atomic Force Microscope / Confocal Laser Scanning Microscope / Endothelial Cell / Mechanical Property / Actin Filament / Shear Stress / Finite Element Method |
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| Research Abstract |
The atomic force microscope (AFM) system was originally developed in combination with an inverted confocal laser scanning microscope to simultaneously measure mechanical stiffness and to observe microstructure of cultured cell. To detect an indentation depth of the cantilever, a cantilever moving system was introduced. A special specimen holder was made to hold a commercially available culture dish. The movements of the cantilever and the specimen holder were controlled by a personal computer. This system was applied to statically cultured and shear stress exposed endothelial cells, and the following results were obtained.
1. Observation of actin filaments and measurement of three dimensional configuration were performed for a fixed endothelial cell.
2. Input and output (I/O) ports of culture medium were newly designed to use a culture dish for flow exposure experiments. Morphology and mechanical properties of cultured endothelial cells were measured using the AFM system. Endothelial cells cultured at static condition had a polygonal shape and more soft mechanical properties around a nucleus than those at peripheral regions. The stiffness of the endothelial cells exposed to shear stress of 2 Pa became higher with the duration time of exposure. Cell shape became elongated to the flow direction and the location of a nucleus moved to downstream side by shear flow.
3. A fluid flow-structural analysis was performed. The three-dimensional finite element model was generated on the basis of the cell surface geometry measured by the AFM. The model consisted of a fluid element and a solid element representing the flow field and the endothelial cells, respectively. Analytical results on stress distribution in the cell showed that high compressive stress appeared mainly in the upstream side. This result may indicate that the stress distributions in the cells have close correlation with the F-actin distributions.
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Research Products
(22 results)
