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2000 Fiscal Year Final Research Report Summary

Analysis of cell-altachment-mutant and isolation of the gene

Research Project

Project/Area Number 10640624
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionUniversity of Tsukuba

Principal Investigator

SATOH Shinoba  Institute of Biological Sciences, Associate Professor, 生物科学系, 助教授 (70196236)

Project Period (FY) 1998 – 2000
Keywordspectin / arabinose / cell adhesion / tobacco / callus / mutant
Research Abstract

When leaf disks from haploid plants of Nicotiana plumbaginifolia Viv. were transformed with T-DNA and cultured on shoot-inducing medium, non-organogenic callus, designated nolac (for non-organogenic callus with loosely attached cells), appeared on-7% of leaf disks. In contrast, normal callus was generated on T-DNA-transformed leaf disks from diploid plants and on non-transformed leaf disks from haploid and diploid plants. Transmission electron microscopy revealed that the middle lamellae and the cell walls of one line of mutant callus (nolac-H14) were barely stained by ruthenium red, even after demethylesterification with NaOH, whereas the entire cell wall and the middle lamella were strongly stained in normal callus. In cultures of nolac-H14 callus, the level of sugar components of pectic polysaccharides in the hemicellulose fraction was reduced and that in the culture medium was elevated, as compared with cultures of normal callus. These results indicate that pectic polysaccharides are not retained in the cell walls and middle lamellae of nolac-H14 callus. In nolac-H14, the ratio of arabinose to galactose (Ara/Gal) was low in the pectic polysaccharides purified from all cell wall fractions and from the medium, in particular, in the hemicellulose fractions. The low levels of T-Ara_f, 5-Ara_f, 2, 5-Ara_f and 3, 5-Ara_f in the pectic polysaccharides of the hemicellulosic fraction of nolac-H14 indicated that no neutral-sugar side chains, composed mainly of linear arabinan, were present in nolac-H14. Arabinose-rich pectins, which are strongly associated with cellulose-hemicellulose complexes, might play an important role in intercellular attachment in the architecture of the cell wall.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] H.Iwai: "High levels of non-methylesterified pectins and low levels of peripherally located pectine in loosely attached non-embryogenic callus of carrot."Plant Cell Report. 18. 561-566 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Iwai: "Production of non-organogenic and loosely attached callus in leaf disk cultures of haploid Nicotiana plumbaginifolia by ^<14>Nion beam irradiation."Plant Biotechnology. 16. 307-309 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Iwai: "High levels of non-methylesterified pectins and low levels of peripherally located pectins in loosely attached non-embryogenic callus of carrot."Planta. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Iwai, A.Kikuchi, T.Kobayashi, H.Kamada, S.Satoh: "High levels of non-methlesterified pectins and low levels of peripherally located pectins in loosely attached non-embryogenic callus of carrot."Plant Cell Reports. 18. 561-566 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Iwai, T.Abe, S.Yoshida, H.Kamada, S.Satoh: "Production of non-organogenic and loosely attached callus in leaf disk cultures of haploid Nicotiana plumbaginifolia by 14N ion beam irradiation."Plant Biotechnology. 16. 307-309 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Iwai, T.Ishii, S.Satoh: "Absence of arabinan in the side chains of the pectic polysaccharides strongly associated with cell walls of Nicotiana plumbaginifolia non-organogenic callus with loosely attached constituent cells."Planta. (In Press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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