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2000 Fiscal Year Final Research Report Summary

Molecular biology of light-responsive genes in cyanobacteria

Research Project

Project/Area Number 10640626
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionThe University of Tokyo

Principal Investigator

IKEUCHI Masahiko  The University of Tokyo Department of Life Sciences Associate Professor, 大学院・総合文化研究科, 助教授 (20159601)

Project Period (FY) 1998 – 2000
Keywordscyanobacteria / blue-greem algae / photosynthesis / high light acclimation / respiration / reactive oxygen species
Research Abstract

Phototrophic organisms respond to various light environments for the purpose of optimization of photosynthesis directly or indirectly. However, the molecular mechanism of light acclimation has not yet been elucidated in detail. We took advantage of transformable cyanobacterium Synechocystis sp.PCC 6803 for molecular analysis of light-responsive mechanism. Genes we have identified in this study are slr2031 (putative Ser/Thr protein phosphatase), spkA (Ser/Thr protein kinase), spkB (Ser/Thr protein kinase), pisJl operon (phytochrome-like photoreceptor and signal transduction pathway genes). We also analyzed high light-responsive genes by using DNA microarray. Through our works, outline of the whole image was given for molecular mechanism with which cyanobacteria respond to light environments.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yoshihara,S. et.al.: "Novel putative photoreceptor and regulatory genes required for the positive..."Plant Cell Physiol.. 41. 1299-1304 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihara,S. et al.: "Mutational analysis of genes involved in pilus structure, motility and..."Plant Cell Physiol.. 42. 63-73 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoh,S. et.al.: "A.Chlorophyll b in expressed in cyanobacteria functions as a light-..."J.Biol.Chem.. 276. 4293-4297 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katoh,H.and Ikeuchi,M.: "Targeted disruption of psbX and biochemical characterization of..."Plant Cell Physiol.. 42. 179-188 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamei,A. et al.: "A eukaryotic-type protein kinase SpkA is required for the normal motility of..."J.Bacteriol.. 183. 1505-1510 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hihara,Y. et al.: "DNA microarray analysis of cyanobacterial gene expression during..."Plant Cell. 13(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hihara,Y. et al.: "A novel gene, pmgA, specifically regulates photosystem..."Plant Physiol.. 117. 1205-1216 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Martinson,T.A. et al.: "Oxygen-evolving diatom thylakoid membranes."Biochim.Biophys.Acta. 1409. 72-86 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umeda,Y. et al.: "Cloning of cellulose synthase genes from Acetobacter xylinum..."DNA Res.. 6. 109-115 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto,S. et al.: "Experimental analysis of recently transposed insertion sequences"DNA Res.. 6. 265-273 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sonoike,K. et al.: "Physiological significance of the regulation of photosystem..."Plant Cell Physiol.. 42(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katoh,H. et al.: "Functional analysis of psbV and a novel c-type cytochrome gene"Plant Cell Physiol.. 42(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihara, S., Suzuki, F., Fujita, H., Geng, X.X.and Ikeuchi, M.: "Novel putative photoreceptor and regulatory genes required for the positive phototactic movement of the unicellular motile cyanobacterium Synechocystis sp.PCC 6803."Plant Cell Physiol.. 41. 1299-1304 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihara, S., Geng, X.X., Okamoto, S., Yura, K., Murata, T., Go, M., Ohmori, M.and Ikeuchi, M.: "Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp.PCC 6803."Plant Cell Physiol.. 42. 63-73 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, S., Ikeuchi, M., Mimuro, M.and Tanaka, A.: "Chlorophyll b in expressed in cyanobacteria functions as a light-harvesting antenna in photosystem I through flexibility of the proteins."J.Biol.Chem.. 276. 4293-4297 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh, H.and Ikeuchi, M.: "Targeted disruption of psbX and biochemical characterization of Photosystem II complex in the thermophilic cyanobacterium Synechococcus elongatus."Plant Cell Physiol.. 42. 179-188 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamei, A., Yuasa, T., Orikawa, K., Geng, X.X.and Ikeuchi, M.: "A eukaryotic-type protein kinase SpkA is required for the normal motility of the unicellular cyanobacterium, Synechocystis sp.PCC 6803."J.Bacteriol.. 183. 1505-1510 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hihara, Y., Kamei, A., Kanehisa, M., Kaplan, A.and Ikeuchi, M.: "DNA microarray analysis of cyanobacterial gene expression during acclimation to high light."Plant Cell. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hihara, Y., Sonoike, K.and Ikeuchi, M.: "A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis sp.PCC 6803 in response to high light."Plant Physiol.. 117. 1205-1216 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Martinson, T.A., Ikeuchi, M.and Plumley, F.G.: "Oxygen-evolving diatom thylakoid membranes."Biochim.Biophys.Acta. 1409. 72-86 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Umeda, Y., Hirano, A., Ishibashi, M., Akiyama, H., Onizuka, T., Ikeuchi, M.and Inoue, Y.: "Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664 : Implication of a novel set of cellulose synthase genes."DNA Res.. 6. 109-115 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, S., Ikeuchi, M.and Ohmori, M.: "Experimental analysis of recently transposed insertion sequences in the cyanobacterium Synechocystis sp.PCC 6803."DBA Res.. 6. 265-273 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sonoike, K.Hihara, Y.and Ikeuchi, M.: "Physiological significance of the regulation of photosystem stoichiometry upon high light acclimation of Synechocystis sp.PCC 6803."Plant Cell Physiol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh, H., Itoh, S., Shen, J.R.and Ikeuchi, M.: "Functional analysis of psbV and a novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus (formerly Synechococcus) elongatus."Plant Cell Physiol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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