2000 Fiscal Year Final Research Report Summary
Molecular analysis of morphogenesis in the basidiomycete Coprinus cinereus.
| Project/Area Number |
10640652
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物形態・構造
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| Research Institution | Okayama University |
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Principal Investigator |
KAMADA Takashi Okayama University, Faculty of Science, Professor, 理学部, 教授 (40033360)
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| Project Period (FY) |
1998 – 2000
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| Keywords | basidiomycete / Coprinus cinereus / clamp connection / sexuality / morphogenesis / mating-type gene |
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| Research Abstract |
Sexual development in the basidiomycete Coprinus cinereus is under the control of the A and B mating type genes. This study aimed at elucidating the gene network acting downstream of the A and B genes with the following results being obtained.
1. In total, 1018 developmental variants in fruiting were induced by mutagenizing oidia of the homokaryotic fruiting strain CopD5-12 with UV light.
2. One of the variants induced, which has a defect in pattern formation in fruiting, was characterized. The variation was found to be due to mutation in the eln2 gene encoding a novel type of microsomal cytochrome P450 enzyme (CYP502).
3. A mutation that blocks clamp formation, an essential step in A-regulated development, was characterized and the clp1 gene responsible for the defect was cloned. clp1 was predicted to encode a novel protein of 365 amino acids. Northern analysis and forced expression of clp1 indicated that expression of clp1 is necessary and sufficient for induction of the A-regulated sexual development.
4. A REMI mutant defective in nuclear migration for dikaryosis, an essential step in B-regulated sexual development, was characterized and the numl gene responsible for the defect was cloned. numl was predicted to encode a protein of 217 amino acids with three leucine zipper motifs.
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Research Products
(8 results)
