1999 Fiscal Year Final Research Report Summary
Effects of symbiotic Chlorella on the circadian rhythms in Paramecium bursaria.
| Project/Area Number |
10640660
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
動物生理・代謝
|
|---|
| Research Institution | IBARAKI UNIVERSITY |
|---|
Principal Investigator |
MIWA Isoji IBARAKI Univ. Fuc. of Science, Professor, 理学部, 教授 (00007816)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | Paramecium bursaria / Symbiotic Chlorella / Circadian rhythm / Mating reactivity / Immaturity |
|---|
| Research Abstract |
The unicellular ciliate Paramecium bursaria contains several hundred cells of the green alga Chlorella established in the cytoplasm as endosymbionts. Cells of P. bursaria show many kinds of circadian rhythms, including mating reactivity and photoaccumulation rhythms. The symbiotic Chlorella forced the Parame-cium cells to lengthen the period and to shift the phase of photoaccumulation rhythm in LL. Furthermore, about the effects of photosynthetic product maltose, the follows were cleared.
1, Maltose forced the white cells to lengthen the period of circadian rhythms in LL.
2, Maltose rescued the mating reactivity rhythms of arrhythmic mutant cells in LL.
3, Maltose were detected in the green cells of day time.
4, Day green cells also contain tri- and tetra-saccharide in the cytosol.
5, Tri-maltose showed the same effect as maltose to circadian rhythms.
On the other hand, cells of P. bursaria have a immature period after conjugation. Correlation of the period length of circadian rhythms with the length of immaturity was examined using a short period mutant cell. Short period cells showed a short immature length. Furthermore, green cells showed a shorter immature length. The question why green cells showed long circadian period was remained.
|
