2000 Fiscal Year Final Research Report Summary
Detection of Biologically Active Substances by Chemical Reception
| Project/Area Number |
10640671
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HANAI Kazumitsu Kyoto Prefectural University of Medicine Professor, 医学部, 教授 (40108642)
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| Project Period (FY) |
1998 – 2000
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| Keywords | chemical sense / Hydra / glutathione response / biologically active peptides / TGF-beta / DBI / computer-assisted image analysis |
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| Research Abstract |
A fresh water coelenterate Hydra shows the feeding behavior after stimulation by reduced glutathione. The behavioral response is modified by a small amount of biologically active substances in the bathing medium. The modification is observed at different stimulatory conditions by each substance. First, we have investigated the cultural conditions under which the modification by biologically active substances is observed reproducibly. We have found salt compositions of the hatching medium, in which prey is hatched, are important for the modification of the hydra response.
Since the hydra behavioral test is so sensitive to biologically active peptides, it is expected to be useful for the investigation of these substances in the biological specimens, which sometimes are available only small amount. We found a TGF-beta activity in the rat cerebrospinal fluid (CSF) of the fatigued rat after forced swimming. In another study, we found the diazepam binding inhibitor (DBI) increased in the rat CSF after quinine stimulation to the oral cavity. We can detect these changes in the biologically active peptides and identify the peptide using this hydra response system easier than with other investigation methods for the detection of biologically active peptides. These examples show usefulness of the hydra response to examine biologically active substances in the small amount of biological samples.
Currently, we examine the hydra response by observing the behavior by a binocular and determine the response. All are human things. This makes the assay to be very difficult to practice. Then we develop a computer-assisted system for the assay. We have completed to develop necessary computer programs to acquire digital images from a CCD camera, to calculate image parameters, and analyze these image parameters.
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[Publications] Li, A-J., Oomura, Y., Sasaki, K., Suzuki, I., Tooyama, K., Hanai, K., Kimura, H., Hori, T.: "A single pre-training glucose injection induces memory facilitation in rodents performing various tasks : Contribution of acidic fibroblast growth factors."Neuroscienc. 85. 785-794 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Inoue, K., Yamazaki, H., Manabe, Y., Fukuda, C., Hanai, K., Fushiki, T.: "Transforming growth factor-beta activated during exercise in brain depresses spontaneous motor activity of animals. Relevance to central fatigue"Brain Res. 846. 145-153 (1999)
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[Publications] Manabe, Y., Yamazaki, H., Fukuda, C., Inoue, K., Fushiki, T.and Hanai, K.: "Suppression of S-methylglutathione-induced tentacle ball formation by peptides and nullification of the suppression by TGF-β in Hydra"Chemical Senses. 25. 173-180 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Manabe, Y., Yamazaki, H., Fukuda, C., Inoue, K., Fushiki, T.and Hanai, K.: "Hydra biological detection of biologically active peptides in rat cerebrospinal fluid"Brain Research Protocols. 5. 312-317 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Manabe, Y., Kuroda, K., Imaizumi, M., Inoue, K., Sako, N., Yamamoto, T., Fushiki, T., Hanai, K.: "A Diazepam-binding inhibitor-like activity in the rat cerebrospinal fluid after stimulation by the quinine-aversive taste."Chemical Sences. 25. 739-746 (2000)
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[Publications] Manabe, Y., Yamazaki, H., Fukuda, C., Inoue, K., Fushiki, T., Hanai, K.: "Determination of TGF-β-like activity in the rat cerebrospinal fluid after exhaustive exercise using anti-TGF-β IgG and the hydra bioassay"Biomedical Research. 21. 191-196 (2000)
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