1999 Fiscal Year Final Research Report Summary
CLASSIFICATION OF JAPANESE PEAR CULTIVARS BY ISOZYME AND DNA ANALYSIS AND CONSTRUCTION OF PHYLOGENETIC TREES
| Project/Area Number |
10660026
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | TOTTORI UNIVERSITY |
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Principal Investigator |
TANABE Kenji FACULTY OF AGRICULTURE, TOTTORI UNIVERSITY, PROFESSOR, 農学部, 教授 (40032106)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hiroyuki FACULTY OF AGRICULTURE, TOTTORI UNIVERSITY, RESEARCH ASSOCIATE, 農学部, 助手 (70283976)
ITAI Akihiro FACULTY OF AGRICULTURE, TOTTORI UNIVERSITY, RESEARCH ASSOCIATE, 農学部, 助手 (10252876)
TAMURA Fumio FACULTY OF AGRICULTURE, TOTTORI UNIVERSITY, ASSOCIATE PROFESSOR, 農学部, 助教授 (50217197)
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| Project Period (FY) |
1998 – 1999
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| Keywords | PYRUS / RELP / O-METHYLTRANSFERASE / ACC SYNTHASE / CHLOROPLAST DNA / FRUIT RIPENING |
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| Research Abstract |
It is unclear whether Japanese pear cultivars (Pyrus pyrifolia Nakai) were imported from China or Korea or improved on Pyrus serotina in Japan. The investigations were conducted to make it clear at molecular level. The maternal relationship in Pyrus species (mainly Japanese pear) was revealed by PCR-RFLP analysis of chloroplast DNA regions. A cDNA clone, JPROMT, was isolated by differential display and its predicted amino acid residues showed high similarity to plant O-methyltransferase. Northern analysis showed that the expression of JPROMT was not observed in ripening fruits of Chinese pear, but in ripening fruits of Japanese pear and European pear. JPROMT was shown to be an useful molecular distinguishing from Japanese, Chinese and European pear. Two 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (PPACS1 and PPACS2) were also shown to be useful markers to classify Japanese pear cultivars.
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