1999 Fiscal Year Final Research Report Summary
Studies on the cloning of few-coiled type of spore in entomopathogenic microsporidia
| Project/Area Number |
10660056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | Nihon University |
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Principal Investigator |
IWANO Hidetoshi Nihon Univ. Bioresource Sci. Assoc. Prof., 生物資源科学部, 助教授 (40120381)
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| Project Period (FY) |
1998 – 1999
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| Keywords | microsporidia / spore dimorphism / cloning / Nosema bombycis / micromanipulator / Vairimorpha necatrix / isolation / cell culture |
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| Research Abstract |
The purpose of this study was to clone entomogenous microsporidia by infecting an insect cultured cell with a single spore. In an attempt to isolate the octospore of Vairimorha necatrix that had been propagated in silkworm larvea reared at 16゜C. In the genus Nusema, one of the two types of the spore, the few-coiled type was recovered from cultured Helicoverpa zea cells infected with Nosema furnacalis and cultured Antheraea eucalypti cells infected with N. bombycis.
A single spore was recovered from each suspension of few-coiled type spores and transferred to a cell culture using a micromanupulator.
Although the developmental stages of N. furnacalis were detected in cultured H. zea cells after 30-day incubation, those of N. bombycis were not. When a single spore of many-coiled type of N. bombycis spore was transferred to cell cultures after alkaline activation, various developmental stages were detected in cultured A. eucalypti cells after 30-day incubation. The cloned microsporidia from a single spore could be subcultured by supplementing the culture with the healthy cells.
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