• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Structural Change and Activity Control of Peptides and Proteins in Biomembranes Including Boundary Lipids

Research Project

Project/Area Number 10660122
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MATSUMURA Yasuki  KYOTO UNIVERSITY, Research Institute for Food Science Associate Prof., 食糧科学研究所, 助教授 (50181756)

Project Period (FY) 1998 – 2000
Keywordsboundary lipids / biomembranes / proteins / enzymes / structural change / activity control
Research Abstract

Acidic phospholipids such as phophatidic acid (PA) and Phosphatidylinositol (PI) and Sphingomyelin (SpM) are believed to act as "boundary lipids" via strong interaction with proteins in biomembranes. These lipids also affect the orientation of phosphatidylcholine (PC) in biomembranes. The purpose of this project is to understand the effects of "boundary lipids" on the structure and activity of peptides and proteins in biomembranes.
In this project, lipoxygenase (LOX) was chosen as a target protein, because this enzyme is known to act at the interface of water and lipid. The effects of addition of boundary lipids on the activity of LOX at the interfaces of emulsion droplets and vesicles were investigated. The activity of LOX was increased dramatically by the addition of PA and fatty acids in vesicle dispersions as well as in emulsions. The kinetic analyses and ζ potential measurements demonstrated that the elevation of LOX activity was due to the increase of adsorptivity of LOX to interfaces through electrostatic interaction. Analyses by dynamic light scattering, electron micrograph and ^<31>P-NMR revealed that the addition of PA and fatty acids induced the formation of hexagonal structure and micellar particles from PC vesicle membranes. These results have shown that "boundary lipids" control the enzymatic activity through modification of membrane structure.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Y.Saka,T.Mori,Y.Matsumura: "Recation of soybean lipoxygenase-3 in emulsions as affected by emulsifiers, salts and phospholipids"Colloid Surfaces B : Biointerfaces. 19. 187-196 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Mizutani,Y.Matsumura et al.: "Factors affecting reaction of cucumber root lipoxygenase in phospholipid vesicle dispersions"Biochim.Biophys.Acta. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Matsumura et al.: "Hydrocolloids 2.Fundamentasl and Applications of Dispersed System in Food, Biology and Medicine"K.Nishinari ed.Elsevier (Amsterdam). 6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.SAKA, T.MORI and Y.MATSUMURA: "Reaction of soybean lipoxygenase-3 in emulsions as affected by emulsifiers, salts and phospholipids."Colloid Surfaces B : Biointerfaces. 19. 187-196 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.MIZUTANI, Y.MATSUMURA, K.MATSUI, K.CHIBA and T.MORI: "Factors affecting reaction of cucumber root lipoxygenase in phospholipid vesicle dispersions."Biochim.Biophys.Acta. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.MATSUMURA, N.MATSUO, J.KIMATA, K.MATSUI and T.MORI: "Reactions of lipoxygenase from cucumber cotyledon in oil-in-water emulsions."Hydrocolloids-part 2 : Fundamentals and Applications in Food, Biology, and Medicine. (ed. by K.NISHINARI) Elsevier (Amsterdam). 57-62 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi