• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

Studies of Okadaic Acid Related Compounds Produced by Bacteria

Research Project

Project/Area Number 10660201
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionKitasato University

Principal Investigator

SATO Shigeru  Kitasato Univerisity, School of Fisheries Sciences, Associate Professor, 水産学部, 助教授 (20170748)

Project Period (FY) 1998 – 1999
Keywordsdiarrhetic shellfish poison / okadaic acid / Prorocentrum / Dinophysis / bacterium / OA antibody / OA-related compound
Research Abstract

The causative compounds of diarrhetic shellfish poisoning (DSP) are okadaic acid (OA) and its derivatives. Dinophysis sp. Are reported to be the causative dinoflagellates of DSP. Prorocentrum lima, a benthic dinoflagellate are also known to possess DSP toxins. Little is known on the biosynthesis of DSP toxins in these dinoflagellates. We obtained each one gram-positive bacterium from D. fortii, a representative species of DSP-causative dinoflagellate and P. lima, after washed with seawater containing formalin. OA nor dinophysistoxin (DTX)-1 were not detected in the cell extracts of these bacteria when analyzed by HPLC, but analysis of the same samples by enzyme linked immnosorbent assay (ELISA) on DSP toxins suggested that unknown OA-related compound(s) are present. In contrast, no reaction with OA antibody was recognized in the cell extracts of other bacterium such as Vibrio sp, Baclius sp nor Esherichia coli. The OA-related compound in the bacteria isolated from the DSP-causative dinoflagellate are water-soluble, relatively high molecule compounds. When the homogenates of bacterial cells were treated with protease, OA-related compounds are recognized in lipid-soluble extract, which are different from OA or its known derivatives such as DTX1 or DTX3. After purification, 7 mg of a compound which reacts with OA antibody was obtained from 35 L culture of a bacterium isolated from P. lima. Further studies are now under progress.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] K. Koike, S. Sato. M. Yamaji. Y. Nagahama, Y. Kotaki, T. Ogata and M. Kodama: "Occurrence of okadaic acid-producing Prorocentrum lima on the Sanriku coast, northern Janap"Toxicon. 36. 2039-2042 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Koike, S.Sato, M.Yamaji, Y.Nagahama, Y.Kotaki, T.Ogata and M.Kodama: "Occurrence of okadaic acid-producing Prorocentrum lima on the Sanriku coast, northern Japan"Toxicon. 36(12). 2039-2042 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi