Characteristics of nutrient absorption in growing calves

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 10660264
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: IWATE UNIVERSITY
• Principal Investigator: ODA Shinichi Iwate University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60211827)
• Co-Investigator(Kenkyū-buntansha): SATOH Jun Iwate University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (30250632) ; ITOH Kikukatsu Iwate University, Faculty of Agriculture, Lecturer, 農学部, 講師 (50232434)
• Project Period (FY): 1998 – 1999
• Keywords: calf / SGLT-1 / glucose absorption / weaning / α-glucosidase / β-galactosidase

Research Abstract

In the small intestine, the brush-border NaィイD1+ィエD1/glucose cotransporters (SGLT-1) play a major role in the absorption of glucose . The objectives of this experiment were to investigate the developmental changes of the intestinal function for glucose absorption and to recognize the assay for SGLT-1 in new born calves. The SGLT-1mRNA was determined by Northern blot analysis . The mucosal SGLT-1mRNA levels were lower in duodenum and gradually increased in proximal intestine before weaning calves. After normal weaning at 5 weeks old , the expression of SGLT-1 in the small intestine was not different between the location of intestine. Changes in α-glucosidase and β-galactosidase activities in the small intestine were also determined. α-glucosidase activity was lower in suckling calves than that of weaned calves , but β-galactosidase activity remained constant during these period.