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1999 Fiscal Year Final Research Report Summary

Studies of molecular mechanisms on the sexual determination and differentiation

Research Project

Project/Area Number 10660268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

SHIMADA Kiyoshi  Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (40065579)

Co-Investigator(Kenkyū-buntansha) SAITO Noboru  Nagoya University, Graduate School of Bioagricultural Scineces, Assistant Professor, 大学院・生命農学研究科, 助手 (40211924)
Project Period (FY) 1998 – 1999
Keywordschicken / estradiol / sexual differentiation / aromatase / anti-Mullerian hormone / in situ hybridization / aromatase inhibitor
Research Abstract

The sex of birds is determined genetically. The bird with homogametic sex (ZZ) is male and has the testis, and the bird with heterogametic sex (ZW) is female and has the ovary. However, there are many unknown mechanisms about the gene expression in related with sexual determination and differentiation. We examined the gene expression of anti-Mullerian hormone (AMH), P450 cl17hydroxylase (P450c17) and P450aromatase (P450arom) in the gonads in chick embryo by in situ hybridization. Although, AMH mRNA was not detected in female gonad. from day 5 of incubation and increased with development, AMH mRNA was not detected in female gonad. P450c17 mRNA was detected in male and female gonads from day 5 of incubation. Although, P450arom mRNA was detected in the female gonad from day 5 of incubation and increased with development, P450arom mRNA was not detected in male gonad. When the female crick was injected with aromatase inhibitor at day 3 of incubation, the level P450arom mRNA was inhibited and the level of AMH mRNA was enhanced. When the male chick was injected with estradiol at day 3 of incubation, the level of AMH mRNA was inhibited and the level of P450arom m RNA was enhanced. These results suggested that AMH and P450arom may be a rival relation for sexual differentiation, and AMH has an important role for male and P450arom has an important role for female.

  • Research Products

    (70 results)

All Other

All Publications (70 results)

  • [Publications] Abinowanto: "Identification of sperm bearing female-specific chromosome in the sex-reversed chicken"J. Exp. Zool.. 280. 65-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kansaku N.: "Effects of protein kinase A inhibitor (H-89) on VIP-and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland"Comp. Biochem. Physiol.. 119C. 89-95 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto K.: "Role of retinoic acid in regulation of mRNA expression of CaBP-D28K in the cerebellum of the chicken"Comp. Biochem. Physiol.. 120A. 237-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kita K.: "Relationship of insulin-like growth factor-I to muscle protein synthesis in fasted chicks"Jpn. Poult. Sci.. 35. 263-270 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furuse M.: "Effects of various N-terminal fragments of glucagon-like peptide-1(7-36) on food intake in the neonatal chick"Brain Res.. 807. 214-217 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kita K.: "Refeeding increase hepatic insuline-like growth factor-I(IGF-I) gene expression and plasma IGF-I concentration in fasted chicks"Brit. Poult. Sci.. 39. 679-682 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki T.: "Changes of AVT levels in plasma, neurohypophysis and hypothalamus in relation to oviposition in the laying hen"Comp. Biochem. Physiol.. 121A. 149-153 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito N.: "Effects of short-term dehydration on plasma osmolality, levels of arginine vasotocin and its hypothalamic gene expression in the laying hen"Comp. Biochem. Physiol.. 121A. 235-239 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada K.: "Gene expression of steroidogenic enzymes in chicken gonads"Journal of Experimental Zoology. 281. 450-456 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furuse M.: "Influence of intracerebroventricular administration of exendin (9-39) on food intake of the newly-hatched chick"Japanese Poultry Science. 35. 376-380 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ieda T.: "Effects of aminoglutethimide on levels of messenger ribonucleic acid of CaBP-D28K and of vitamin D3 receptor in the shell gland of laying hen"Japanese Poultry Science. 35. 346-355 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirabayashi N.: "Toxicokinetic study of Fadrozole, an aromatase inhibitor, in the chicken Eggs by the injection on the shell membrane"Japanese Poultry Science. 36. 382-387 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島田 清司: "雄と雌が決まる仕組み;魚から鳥、哺乳類まで"生命誌. 7. 4-9 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bungo T.: "Effects of naloxone on the anorexic action by glucagon-like peptide-1(7-36) in the neonatal chick"Japanese Poultry Science. 36. 109-115 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furuse M.: "Central gastrin inhibits feeding behavior and food passage in neonatal chicks"Life Sciences. 65. 305-311 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bungo T.: "Intraventricular injection of glucagon-like peptide-1 inhbits water intake of the neonatal chick"Japanese Poultry Science. 36. 229-235 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito N.: "Gene expression of arginin vasotocin in ovarian and uterine tissues of the chicken"Asian-Australasian Journal of Animal Science. 12. 695-701 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito N.: "Effects of naloxone on neurohypophysial peptides release by hypertonic stimulation in chicks"General and Comparative Endocrinology. 115. 228-235 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bungo T.: "Influence of intracerebroventricular administration of morphine and kyotrophin on food intake of the neonatal chick"Japanese Poultry Science. 36. 319-324 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ieda T.: "Effect of low calcium diet on messenger ribonucleic acid levels of calbindin-D28K of intestine and shell gland in laying hens in relation to egg shell quality"Japanese Poultry Science. 36. 295-303 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bungo T.: "-E)ffects of substitution of N-terminal amino acid of glucagon-like peptide-(7-36) amide on food intake of the noenatal chick"Life Sciences. 25. PL295-PL299 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bungo T.: "Intracerebroventricularly administration of glucagon-like peptide-1 induces sleeplike behavior in the neonatal chick"Japanese Poultry Science. 36. 377-381 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing hormone receptor, P450 17α-hydroxylase and P450 aromatase in sdolated grnulosa, theca interna and theca externa layers of chicken ovarian follicles during follicular growth"Animal Science Journal. 70. 12-17 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishikimi H.: "Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken"Molecular Reproduction and Development. 55. 20-30 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tan F.-I.: "Molecular cloning and functional charactaerization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken"Biology of Reproduction. 62. 8-15 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T.: "Cytogenetical mapping of thirty-one functional genes on chicken chromosomes by the direct R-banding FISH"Cytogenetics Cell Genet.. 87. 32-40 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T.: "Cytogenetical assignment of 29 functional genes on chicken microchromosomes by FISH"Cytogenetics Cell Genet.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T.: "Comparative FISH mapping on Z chromosomes of chicken and Japanese quail"Cytogenetics Cell Genet.. 87. 22-26 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki T.: "Regulation of opioid peptides on the release of arginine vasotocin in the hen"Journal of Experimental Zoology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ieda T.: "Changes in parathyroid hormone related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle"General and Comparative Endocrinology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada, K.: "Development and Differentiation of Neuroendocrine System : Novel Answer to Classic Questions"Tokyo Metropolitan Institute for Neuroscience. 121 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada K.: "Reproductive Biology Update-novel tools for assessment of environmental toxicity"Shoukadoh Booksellers Company. 498 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada K.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikuchi M.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito N.: "Recent Progress in Molecular and Comparative Endocrinology"Hormone Research Center. 625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kansaku N.: "Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolaction and growth hormone in the chicken pituitary gland"Comp. Biochem. Physiol.. 119C. 89-95 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto K.: "Role of retinoic acid in regulation of mRNA expression of CaBP-D28K in the cerebllum of the chicken"Comp. Biochem. Physiol.. 120A. 237-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kita K.: "Relationship of insulin-like growth factor-I to muscle protein synthesis in fasted chicks"Jpn. Poult. Sci.. 35. 263-270 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furuse M.: "Effects of various N-terminal fragments of glucagon-like peptide-1(7-36) on food intake in the neonatal chick"Brain Res.. 807. 214-217 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kita K.: "Refeeding increases hepatic insuline-like growth factor-I(IGF-I) gene expression and plasma IGF-I concentration in fasted chicks"Brit. Poult. Sci.. 39. 679-682 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abinawanto: "Identification of sperm bearing female-specific chromosome in the sex-reversed chicken"Journal of Experimental Zoology. 280. 65-72 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki T.: "Changes of AVT levels in plasma, neurohypophysis and hypothalamus in relation to oviposition in the laying hen"Comparative Biochemictry and Physiology. 121A. 149-153 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito N.: "Effects of short-term dehydration on plasma osmolality, levels of arginine vastocin and its hypothalamic gene expression in the layinghen"Comparative Biochemictry and Physiology. 121A. 235-239 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ieda T.: "Effects of aminoglutethimide on levels of messemger ribonucleic acid of CaBP-D28K and of vitamin D3 recepteor in the shell glandof laying hen"Japanese Poulty Science. 35. 346-355 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furuse M.: "Influence of intracerebroventricular administration of exendin (9-39) on food intake on the newly-hatched chick"Japanese Poulty Science. 35. 376-380 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada, K.: "Gene expression of steroidogenic enzymes in chicken gonads"Journal of Experimental Zoology. 281. 450-456 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing hormone receptor, P450 17α-hydroxylase and P450 aromatase in isolated granulosa, theca interna and theca externa layers of chicken ovarian follicies during follicular growth"Animal Science Journal. 70. 12-17 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bungo T.: "Effects of naloxone on the anorexic action by glucagon-like peptide-1 (7-36) in the neonatal chick"Japanese Poulty Science. 36. 109-115 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furuse M.: "Central gastrin inhibits feeding behavior and food passage in neonatal chicks"Life Science. 65. 305-311 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bungo T.: "Intraventricular injection of glucagon-like peptide-1 inhibits water intake of the neonatal chick"Japanese Poulty Science. 36. 229-235 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito N.: "Gene expression of arginine vasotocin in ovarian and uterine tissues of the chicken"Asian-Australasian Journal of Animal Science. 12. 695-701 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito N.: "Effects of naloxone on neurohypophysial peptides release by hypertonic stimulation in chicks"General and Comparative Endocrinology. 115. 228-235 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bungo T.: "Influence of intracerebroventricular administration of morphine and kyotrophin on food intake of the neonatal chick"Japanese Poulty Science. 36. 319-324 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ieda T.: "Effect of low calcium diet on messenger ribonucleic acid levels of cabindin-D28K of intestine and shell gland in laying hens in relation to egg shell quality"Japanese Poulty Science. 36. 295-303 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bungo T.: "Effects of substitution of N-terminal amino acid of glucagon-like peptide-1 (7-36) amide on food intake of the neonatal chick"Life Sciences. 25. Pl295-Pl299 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bungo T.: "Intracerebroventricularly administration glucagon-like peptide-1 induces sleep-like behavior in the neonatal chick"Japanese poulty Science. 36. 377-381 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirabayashi N.: "Toxicokinetic study of Fadrozole, an aromatase inhibitor, in the chicken Eggs by the injection on the shell membrane"Japanese poulty Science. 36. 382-387 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada K.: "The mechanism of the sexual determination ; from fish to mammal"Biohistory. 7. 4-9 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tan F.-I.: "Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken"Biology of Reproduction. 62. 8-15 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikimi H.: "Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken"Molecular Reproduction and Development. 55. 20-30 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki T.: "Comparative FISH mapping on Z chromosomes of chicken and Japanese quail"Cytogenetics Cell Genet.. 87. 22-26 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki T.: "Cytogenetical mapping of thirty-one functional genes on chicken chromosomes by the direct R-banding FISH"Cytogenetics Cell Genet.. 87. 32-40 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki T.: "Cytogenetical assignment of 29 functional geens on chicken microchtomosomes by FISH"Cytogenetics Cell Genet.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ieda T.: "Changes in parathyroid hormone related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle"General and Comparative Endocrinology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki T.: "Regulation of opioid peptides on the release of arginine vasotocin in the hen"Journal of Experimental Zoology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada K.: "Reproductive Biology Update-novel tools for assessment of environmental toxicity"Shoukadoh Booksellars Company. 495. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada K.: "Development and differentiation of Neuroendocrine System : Novel Answers to Classic Questions"Tokyo Metropolitan Institute for Neuroscience. 121. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada K.: "Progress in Molecular and COnparative Endorinology"Hormone Research Cneter. 625. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito N.: "Progress in Molecular and COnparative Endorinology"Hormone Research Cneter. 625. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikuchi M.: "Progress in Molecular and COnparative Endorinology"Hormone Research Cneter. 625. (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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