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2000 Fiscal Year Final Research Report Summary

Contribution of Collagen to the development of mouse early embryo

Research Project

Project/Area Number 10660276
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNippon Veterinary and Animal Science University

Principal Investigator

TOMOGANE Hiroshi  Nippon Vet & Anim Sci Univ Professor, 獣医畜産学部, 教授 (30023493)

Co-Investigator(Kenkyū-buntansha) OHBOSHI Shigeki  Nippon Vet & Anim Sci Univ Lecturer, 獣医畜産学部, 講師 (10277668)
Project Period (FY) 1998 – 2000
Keywordsmouse fertilized ovum / collagen / cis-4-hydroxy-2-proline / collagen gene / morula / blastocyst
Research Abstract

Study(1) : A fertilized ovum repeats cell divesion cycles to increase the number of cell. The increased cell forms morula and blastocyst. In all tissues there is always collagen as extracellular matrix. To determine the contribution of collagen to the development of preimplantation embryos, cis-4-hydroxy-2-proline (CHP), collagen sysnthesis inhibitor, was added into the medium for the in vitro development of mouse 2-cell embryo and the in vitro development of 2-cell stage to blastocyst was estimated by the developmental rate to 4-cell, 8-cell, morula and blastocyst and by the number of cell at the developed blastocyst. The CHP addition blocked to develop to 4-cell satge from 2-cell stage. This blocking effect was inhibited by the replacement of proline at the same of CHP addition but not the replacement of lysine or glycine. Study(2) : The blocking effect of CHP on in vitro embryonic development was examined at various stages of embryos. The blocking occurred remarkably at the stages of from 2-cell to 4-cell and from morula to blastocyst. Study(3) : Using reverse transeription polymerase chain reaction method, mRNA of type I and III collagen weakly appeared at the stage of 4-cell. The typelV collagen mRNA first appeared from 8-cell stage and more at the blastocy. Study(4) : The collagen at the various stages of preimplantation embryos was determined using immuno-fluoresence- histochemistry. The collagen immuno-reaction was weakly detected at 2-cell, 4-cell and 8-cell stages and strongly at both morula and blastocyst cmbryos. These results indicate that collagen has important role for the early embryonic development and is essential subustance to develop to 4-cell stage and to blastocyst from morula.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kawai,Yasuhiro: "Comparison of the extent of DNA damage in rat embryos developed in vivo and in vitro."Animal Science Journal. 71(4). 425-427 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohboshi,Shigeki: "Cryopreservation of mammalian embryos by vitrification."Animal Science and Technology(Jpn). 69(12). 1069-1077 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawai, Yasuhiro: "Comparison of the extent of DNA damage in rat embryos developed in vivo and in vitro."Animal Science Journal. 71(4). 425-427 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohboshi, Shigeki: "Cryopreservation of mammalian embryos by vitrification."Animal Science and Technology (Jpn). 69(12). 1069-1077 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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