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1999 Fiscal Year Final Research Report Summary

CANINE OSTEOSARCOMA CELLS EXPRESS MMP-9 THROUGH CELL-MATRIX

Research Project

Project/Area Number 10660280
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionTokyo University of Agriculture & Technology

Principal Investigator

ARAI Katsuhiko  Tokyo University of Agriculture and Technology, Department of Tissue Physiology, Associate Professor, 農学部, 助教授 (60175940)

Project Period (FY) 1998 – 1999
KeywordsMMP / TUMOR / INTEGRIN / SIGNAL TRANSDUCTION
Research Abstract

To investigate the participation of MMP-9 in the formation of lung metastasis, we have established an animal model for lung metastasis by the injection of osteosarcoma (OS) cells into mammary fad pad of SCID mice. Gelatinzymography revealed a great deal of MMP-9 secreted by the lung tissues containing metastatic lesions, whereas only MMP-2 was detected in 'hypothetic' origin these results suggested that OS cells could acquire the ability of MMP-9 expression during tumor invasion and metastasis. Therefore, we examined the mechanism of MMP-9 expression under several culture condition in vitro. In monolayer culture of OS cells on several extracellular matrix, MMP-9 was not detected by gelatin zymography, while MMP-2 was constantly expressed. When OS cells were cultivated in type I collagen gel, MMP-9 was apparently detected in addition of MMP-2. The expression of MMP-9 in collagen gel was down-regulated by anti-integrin antibodies and cytochalasin D. Thus, we suggested that the expression of MMP-9 by OS cells was regulated through integrin-mediated cell-matrix interaction and following actin polymerization.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] A. TANAKA et al.: "Matrix metalloproteinase -9 production, a newly identified function of mast cell progenitors, is downregulated by c-kit activation"Blood. 94. 2390-2395 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新井 他: "細胞外マトリックス研究法全6巻"コラーゲン技術研修会. 789 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. TANAKA, K. ARAI.,Y. KITAMURA and H. MATSUDA: "Matrix metalloproteinase-9 production, a newly Identified function of mast cell progenitors, is downregulated by c-kit receptor activation"Blood. 94. 2390-2395 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima K, Kishi H, Itoh M, Watanabe G, Arai K, Uehara K, Groome NP, Taya K: "Secrtion of inhibin A, inhibin B and inhibin pro-alphaC during the oestrous cycle of the golden hamster (Mesocricetus auratus)"J. Endocrinol. 162. 451-456 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. ARAI., K. OHSHIMA, G. WATANABE, K. ARAI, K. UEHARA, K. TAYA: "Inhibition of ovarian follicular developed associated with a decrease in luterinizing hormone levels during the estrous cycle of the rat"Endocrine J., 1998. 45. 539-546 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. ARAI., A. KIMURA, K. ARAI, K. UEHARA and R. HATA: "Fine structural analysis of the unique 5'region of human COL1A2 gene containing two regions of dinucleotide repeats adjacent to the reansciptional start site"Connective Tissue. 30. 1-6 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. ARAI., T. SHIRAI, S. HATTORI: "Gelatin zymography"Methods for Extracellular Matrix Research VOL. 2 (Eds. R. HATA, S. HATTORI and K. ARAI). 78-88 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. ARAI: "Non-isotopic in situ hybridization"Methods for Extracellular Matrix Research VOL. 6 (Eds. R. HATA, S. HATTORI and K. ARAI). 78-88 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. ARAI: "Immunohistochemistry"Methods for Extracellular Matrix Research VOL. 6 (Eds. R. HATA, S. HATTORI and K. ARAI). 17-30 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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