1999 Fiscal Year Final Research Report Summary

Pathogenicity and molecular structer of a newly isolated lethal and broad host range herpesvirus

Research Project

Project/Area Number	10660299
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied veterinary science
Research Institution	Gifu University Faculty of Agriculture
Principal Investigator	FUKUSHI Hideto Gifu University Dept. Vet. Microbiol., Associate Professor, 農学部, 助教授 (10156763)
Co-Investigator(Kenkyū-buntansha)	MATSUMURA Tomio JRA, Lab. Mol. Biol. Senior Researcher, 競走馬総合研究所・栃木支所・分子生物学研究室, 研究役 YAMAGUCHI Tsuyoshi Gifu University Dept. Vet. Microbiol., Assistant Professor, 農学部, 助手 (70210367)
Project Period (FY)	1998 – 1999
Keywords	herpesvirus / neuropathogenicity / hamster
Research Abstract	We investigated the pathogenicity of EHV-9 in horses. The horse inoculated with EHV-9 showed pyrexia, verifier and viral excretion in nasal discharge without any neurological symptoms. However, nonsuppurative encephalitis was confirmed. These results indicated that EHV-9 using hamsters. We serially passaged EHV-9 in the rabbit kidney cell line, RK-13. After 23 passages, we observed smaller plaques of EHV-9 in MDBK cells. This small plaque forming EHV-9 culture has less pathogencity in hamsters. We cloned the small plaque forming EHV-9. The cloned viruses also showed less pathogencity in hamsters. These results indicated that the plaque sizes in MDBK cells are related to the pathogenicity in hamsters.

(4 results)