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2000 Fiscal Year Final Research Report Summary

STUDY OF THE STRUCTURE AND FUNCTION OF HETEROTRIMERIC G PROTEINS IN PLANTS

Research Project

Project/Area Number 10660318
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionFUKUI PREFECTURAL UNIVERSITY

Principal Investigator

IWASAKI Yukimoto  FUKUI PREFECTURAL UNIVERSITY, BIOSCIENCE, ASSOCIATE PROFESSOR, 生物資源学部, 助教授 (20193732)

Co-Investigator(Kenkyū-buntansha) ASAHI Tadashi  FUKUI PREFECTURAL UNIVERSITY, BIOSCIENCE, PROFESSOR, 生物資源学部, 教授 (10023392)
Project Period (FY) 1998 – 2000
KeywordsGibberellin / G protein / GUS / Promoter / Rice
Research Abstract

Transgenic rice containing an antisense cDNA for the α subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the α subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the α-subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the α subunit by antisense technology.
The chimera gene containing the promoter region of the gene of the α subunit of a heterotrimeric G protein (RGA1) and GUS gene, was introduced to the normal cultivar and the seeds at third generation (T3 seeds) were used for further analysis. In germination stage, the GUS activity was detected in aleurone layer, scutellar epithelium and leaf sheath. In the developing stage, it was largely observed in the internodes. Before heading, GUS activity was localized in the lemma and palea. After heading, it was detected in pollen. The expression of the α subunit in leaf sheath, internode, lemma and palea is accountable for explanation of abnormal phenotypes in Daikoku d1.
Rice dwarf mutant, d1, is defective in the α subunit of the heterotrimeric G protein. Compared with the wild type, gibberellin induction of α-amylase activity in aleurone cells of d1 was greatly reduced. This result shows that the heterotrimeric G protein concern the gibberellin signal transduction.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Tanaka-Ueguch,M. et al.: "Rice dwarf mutant, dl, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction"Proc.Natl.Acad.Sci.USA. 97. 11638-11643 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,T. et al: "Molecular cloning and characterization of cDNA for a rice Sec31p homolog"Biosci.Biotechnol.Biochem.. 64. 2490-2492 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanabe S.B. et al: "High resolution map of rice dwarf mutant gene, dwarf 11."Rice Genetics Newsletter. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka-Ueguchi, M., Fujisawa, Y., Kobayashi, M., Ashikari, M., Iwasaki, Y., Kitano, H.and Matsuoka, M.: "Rice dwarf mutant, d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction."Proc.Natl.Acad.Sci.USA.. 97. 11638-11643 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, T., Ishikawa A., Asahi, T.and Iwasaki, Y.: "Molecular cloning and characterization of a cDNA for a rice Sec31p homolog."Biochi.Biotechnol.Biochem.. 64. 2490-2492 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanabe, S., Ashikari, M., Yano, M., Yoshimura, A., Matsuoka, M., Kitano, H., Asahi, T.and Iwasaki, Y.: "High resolution map of rice dwarf mutant gene, dwarf 11."Rice Genetics Newsletter. 17. 18-19 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujisawa, Y., Kato T., Ohki, S., Ishikawa, A., Kitano, H., Sasaki, T., Asahi, T.and Iwasaki, Y.: "Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice."Proc.Natl.Acad.Sci.USA. 96. 7575-7580 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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