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2000 Fiscal Year Final Research Report Summary

Resolution of relationship between expression of water channel genes and rice chilling tolerance

Research Project

Project/Area Number 10660319
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionAkita Prefectural University (1999-2000)
Akita Prefectural College of Agriculture (1998)

Principal Investigator

KITAGAWA Yoshichika  Akita Prefectural University, Professor, 生物資源科学部, 教授 (10006752)

Project Period (FY) 1998 – 2000
Keywordswater channels / water stress / chilling tolerance / rice
Research Abstract

(1)Relationship between water channel genes and chilling tolerance : Three water channel genes (rwc1, rwc2 and rwc3) has been isolted from cDNA library of chilling sensitive rice variety, Wasetoitsu. The rwc3 is specific expressed in rice roots and has functionally water absorption into roots under chilling condition.. when chilling tolerant rice seedlings was chilled, rwc3 was expressed and rice seedlings were not died ; however, chilling tolerant rice seedlings were died and rwc3 was disappeared when the seedlings were treated with caffeine which was calcium channel blocker. These results suggest closed relationship between expression of water channel genes and rice chilling tolerance.
(2)Another analysis : Promoter sequence (4 kbp) of rwc3 was analysied. The unique sequences were found in this sequence. To analysis promoter function under induction of chilling tolerance, the repoter gene, GUS, was connected under rwc3 promoter and introduced in rice cells by particle gun.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Joshee, N.et al.: "Isolation and characterization of a water stress-specific genomic gene, pwsi18, from rice."Plant Cell Physiol.. 39(1). 64-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitagawa, Y.et al.: "Water stress-induced chilling tolerance in rice ; putative relationship between chilling tolerance and Ca2+ flux."Plant Sci.. 173. 64-85 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li, L et al.: "Molecular cloning of a novel water channel from rice : its products expression in Xenopus oocytes and involvment in cilling tolerance."Plant Sci.. 154. 43-51 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Joshee, N., Kitagawa, Y.: "Isolation and characterization of a water stress-specific gene, pwsi18, from rice : possible regulatory role of putative cis-acting elements present in the promoter."Plant Cell Physiol.. 39. 64-72 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitagawa, Y., Joshee, N., Yoshizaki, Y.: "Water stress-induced chilling tolerance in rice : Putative relationship between chilling tolerance and Ca^<2+> flux."Plant Sci.. 137. 73-85 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li, L., L, S., Tao, Y., Kitagawa, Y.: "Molecular cloning of a novel water channel from rice : its products expression in Xenopus oocytes and involvement in chilling tolerance."Plant Sci.. 154. 43-51 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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