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1999 Fiscal Year Final Research Report Summary

The moelcular basis of the mechanisums on that the extracellular calcium ions regulate the differentiation of human esopahgeal epithelial cells

Research Project

Project/Area Number 10670009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

HITOMI Jiro  School of Medicine, NIIGATA UNIVERSITY Associate Professor, 医学部, 助教授 (00218728)

Project Period (FY) 1998 – 1999
Keywordscalcium signaling / squamous epithelial cells / differentiation / calcium-binding proteins / CAAF1 / S100A12 / S100P / esophagus
Research Abstract

Extracellular calcium ions (Ca^<2+>) are important in regulating the differentiation of squamous epithelial cells. We tried to clarify the mechanisms of the differentiation of human esophageal epithelial cells using the primary culture of human esophageal epithelial cells (EECs), which can be differentiated by increasing the concentration of extracellular Ca^<2+>. And we got three new findings as the below :
1) A calcium-binding protein S100P showed a Ca^<2+> inducible expression in human EECs. S100P was detected in vivo in the suprabasal and the upper few layers of the epithelium, in which the proliferating cells were also located. S100P expression is transiently expressed during the early stage of differentiation.
2) Human esophageal epithelial cells sequentially produce different S100 proteins, including S100A2, S100A6, S100P and CAAF1 (S100A12) in the process of differentiation.
3) Human CAAF1 proteins exist as noncovalent homodimers, and Ca^<2+> and Zn^<2+> synergistically promote the oligomeric self-association.
These findings suggest that within esophageal epithelial cells S100 proteins might play important roles in cell differentiation during specific stages. An understanding of calcium function in the squamous epithelial cells will require the analysis of calcium-binding proteins, especially S100 proteins.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 佐藤信之: "S100P expression in human esophageal epithelial cells : Human esophageal epithelial cells sequentially produce different S100 proteins in the process of terminal differentiation"Anatomical Record. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATO,N.and HITOMI,J.: "S100P expression in human esophageal epithelial cells : Human esophagel epithelial cells sequentially produce different S100 proteins in the process of terminal differentiation"Anatomical Record. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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