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2000 Fiscal Year Final Research Report Summary

Response system to mechanical stretching in the vascular endothelial cells

Research Project

Project/Area Number 10670033
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNippon Medical School

Principal Investigator

SUGIMOTO Keijii  Nippon Medical School, Faculty of medicine Associate professor, 医学部, 助教授 (20104002)

Co-Investigator(Kenkyū-buntansha) TAKEMASA Tohru  University of Tsukuba, Institute of Health and Sports Sciences Lecturer, 体育科学系, 講師 (50236501)
YAMASHITA Kazuo  Nippon Medical School, Faculty of medicine Professor, 医学部, 教授 (70022796)
Project Period (FY) 1998 – 2000
KeywordsEndothelial cells / Tensile stress / Cytoskeleton / Stress fibers / Molecular chaperone / Mechanical stress / Nitric oxide / Heat shock protein 70
Research Abstract

Histological quantitative study on the formation of stress fibers (SF) and expression of heat shock protein (HSP) in endothelial cells in response to mechanical stretching was performed using excised rat arterial beds. They were classified into two groups in respect of inductivity of these parameters. In order to examine the effects of such stress on the functions of endothelial cells, histochemical detection of intracellular nitric oxide (NO) in fixed tissues was attempted. We found that a combination of aldehyde fixatives with 4, 5-diaminoflurescein diacetate is useful for this purpose. Using this NO detecting system, NO production activity in endothelial cells in vivo will be examined in near future study after loading some kinds of stresses.
On the other hand, the relationship between SF formation and HSP expression in endothelial cells loaded mechanical stretching was also analyzed. While inductivity of HSP25 and HSP90 in the cells in response to the stress was relatively low, HSP70 expression was evidently stimulated in the cells applied the stress. Furthermore, it has been demonstrated that the inhibition of HSP70 expression by treatment of the cells with quercetin resulted in suppression of SF formation. However, inhibition of SF formation by ttreatment of the cells with cytochalasin D had no effect on HSP70 expression. Thus, HSP70 probably acts as a molecular chaperone in SF formation in endothelilal cells in response to mechanical stretching.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Tohru Takemasa: "Oblique alignment of stress fibers in cells reduces the mechanical stress in cyclically deforming fields."Eur.J.Cell Biol.. 77. 91-99 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] hajime Kameya: "Morphological and functional changes in rat endothelial cells after ischemia-reperfusion."Biomed.Res.. 19. 369-379 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉本啓治: "血管内皮における張力刺激と細胞骨格"生体の科学. 49. 93-98 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 武政徹: "シリコンベルトを使った培養細胞伸縮装置の開発"生物物理. 38. 68-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiji Sugimoto: "Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate."Histochem.Cell Biol.. 113. 341-347 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉本啓治: "血管内皮における機械的刺激によるストレスタンパク質の発現"生体の科学. 51. 562-566 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohru Takemasa, Takami Yamaguchi, Yasuhito Yamamoto, Keiji Sugimoto, and Kazuo Yamashita: "Oblique alignment of stress fibers in cells reduces the mechanical stress in cyclically deforming fields."Eur.J.Cell Biol.. 77. 91-99 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hajime Kameya, Keiji Sugimoto, Shigeo Tanaka, and Kazuo Yamashita: "Morphological and functional changes in rat endothelial cells after ischemia-reperfusion."Biomed.Res.. 19. 369-379 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa. and Kazuo Yamashita: "Cytoskeleton changes in response to tensile stress in vascular endothelial cells."SEITAI NO KAGAKU. 49(2). 93-98 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tohru Takemasa, Keiji Sugimoto, and Kazuo Yamashita: "Silicone belt system for cyclic stretching of cultured cells."BIOPYSICS. 38. 68-71 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tohru Takemasa, Keiji Sugimoto, and Kazuo Yamashita: "oblique alignment of stress fibers in the cyclically strained cells can be deduced from the principle of minimum potential energy."Jpn.J.Biochem.Exercise. 10. 55-57 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa, and Kazuo Yamashita: "Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate."Histochem.Cell Biol.. 113. 341-347 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa. and Kazuo Yamashita: "Expression of stress proteins in vascular endothelial cells in response to mechanical stress."SEITAL NO KAGAKU. 51(6). 562-566 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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