1999 Fiscal Year Final Research Report Summary
Functional Analysis of a Novel Imprinted Gene Impact : Multiplexed Approach using Various Model Organisms
| Project/Area Number |
10670113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Takashi The University of Tokyo, Institute of Medical Science, Associate Professor, 医科学研究所, 助教授 (90201326)
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| Project Period (FY) |
1998 – 1999
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| Keywords | imprinting / Impact / model organism / methylation |
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| Research Abstract |
(1) Analysis of Yeast Impact Homolog 1 (YIH1)
Yih 1 has a novel protein-binding module termed GI motif, which is also found in the yeast eIF2α-kinase Gcn2. The GI motif-mediated binding of Gcn2 to Gcn1 is necessary for the activation of the former by the latter, and Yih1 can compete with Gcn2 for Gcn1, thereby inhibiting the phosphorylation of eIF2, a key step for the regulation of translation.
(2) Analysis of Nematoda homolog of Impact
We cloned the cDNA for the nematoda homolog of Impact, and used it for RNAi to fail to observe any apparent phenotypes.
(3) Analysis of Xenopus homolog of Impact
We examined the allelic expression of Xenopus Impact using the F I hybrid frog between X.laevis and X.borealis, and showed that it is expresssed biallelically. Microinjection of Ximpact mRNA causes gasturulation defects.
(4) Analysis of human IMPACT and mouse Impact
We cloned human IMPACT and found that it is not imprinted in human. We thus elucidated the genome sequences for both mouse and human genes and revealed that a characteristic repeat-containing CpG island is found in mouse, but not in human. Furthermore, we found that the island is subjected to parent-of-origin-dependent methylation.
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Research Products
(16 results)
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[Publications] Mizuki, K., Kadomatsu, K., Hata, K., Ito, T., Fan, Q.-W., Kage, Y, Fukumaki, Y., Sakaki, Y., Takeshige, K. & Sumimoto, H.: "Functional modules and expression of mouse p40ィイD1phoxィエD1 and p67ィイD1phoxィエD1 Src homology 3 (SH3) domain-containing proteins involved in the phagocyte NADPhoxidase complex"Eur. J. Biochem.. 251. 573-582 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, R., Sumimoto, H., Mizuki, K., Hata, K., Ago, T., Kitajima, S., Takeshige, K., Sakaki, Y. & Ito, T.: "The PC motif : a novel and evolutionarily conserved sequence involved in interaction between p40ィイD1phoxィエD1 and p67ィイD1phoxィエD1, SH3 domain-containing cytosolic factors of the phagocyte NADPhoxidase"Eur. J. Biochem.. 251. 583-589 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Oka, M., Furihata, C., Kitoh, K., Yamamoto, M., Tatematsu, M., Ichinose, M., Miki, K., Ito., Sakaki, Y. & Reske, K.: "Dendritic cell response and susceptibility to stomach caused by N-methyl-N'-nitro-N-nitroso-guanidine in rats"Cancer Res.. 58. 4107-4112 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamada, Y., Hagiwara, Y., Shiokawa, K., Sakaki, Y. & Ito, T.: "Spatiotemporal, allelic, and enforced expression of Ximpact, the Xenopus homolog of mouse imprinted gene Impact"Biochem. Biophys. Res. Commun.. 256. 162-169 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Ito, T., Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K., Kuhara, S. & Sakaki, Y.: "Toward a protein-protein interaction map of the budding yeast : a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins"Proc. Natl. Acad. Sci. USA. 97. 1143-1147 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Iijima, Y., Ito, T., Oikawa, T., Eguchi, M. Eguchi-Ishimae, M., Kamada, N., Kishi, K., Asano, S. Sakaki, Y. & Sato, Y.: "A new ETV6/TEL partner gene, ARG (ABL related gene or ABL2), identified in an AML-M3 cell line with a t(1 ; 12)(q25 ; pl3) translocation"Blood. (in press).
Description
「研究成果報告書概要(欧文)」より
